A rapid and specific fluorescent activity staining procedure for transamidating enzymes.
A rapid and specific fluorescent activity staining procedure for transamidating enzymes.
复制标题
用于转酰胺基酶的快速且特异的荧光活性染色程序。
DOI：
10.1016/s0003-2697(79)80177-3
复制
发表时间：
1979
影响因子：
2.9
通讯作者：
J. Stenflo
中科院分区：
文献类型：
--
作者：
P. Stenberg;J. Stenflo
已经开发了一种快速、灵敏和特异的方法用于检测转氨酶（转氨酶，例如，因子XIII）。该技术是基于转酰胺酶催化掺入的荧光monodansylthiacadaverine到酪蛋白。高灵敏度可用于检测和表征血浆、血小板、红细胞裂解物和组织提取物中的转氨酶。该技术也可以与交叉免疫电泳相结合。
A rapid, sensitive, and specific procedure has been developed for detection of transsamidatingenzymes (transglutaminase, e.g., factor XIII) after agarose gel electrophoresis. The technique is based on the transamidase-catalyzed incorporation of the fluorescent monodansylthiacadaverine into casein. The high sensitivity enables detection and characterization of transamidases in blood plasma, platelet, and red blood cell lysate and tissue extracts. The technique can also be combined with crossed immunoelectrophoresis.