Miniature probe for all-optical double gradient-index lenses photoacoustic microscopy
Miniature probe for all-optical double gradient-index lenses photoacoustic microscopy
复制标题
用于全光学双梯度折射率透镜光声显微镜的微型探头
DOI：
10.1002/jbio.201800147
复制
发表时间：
2018-12-01
影响因子：
2.8
通讯作者：
Chen, Sung-Liang
中科院分区：
文献类型：
--
作者：
Guo, Zhendong;Li, Guangyao;Chen, Sung-Liang
提出了一种新型的全光学双梯度折射率(GRIN)透镜光学分辨率光声显微镜(OR-PAM)，称为DGL-PAM。该微型探头由用于光学聚焦的单模光纤和双梯度折射率透镜以及用于超声检测的微型光纤法布里-珀罗传感器组成。新的设计简单，通过双GRIN透镜实现了长工作距离(WD)的高分辨率。探头的总尺寸为2.7毫米直径。获得了3.7微米的横向分辨率(532 nm激光波长)和5.5 mm的长WD。在体小鼠耳的OR-PAM显示了DGL-PAM的成像能力。由于不需要光学和声学焦点的精确对准，所提出的DGL-PAM相对容易实现。它有可能被开发为一种低成本、一次性的OR-PAM探针，并用于内窥镜检查。所提出的用于制造微型内窥镜探头的双GRIN透镜也可以应用于其他方式，例如光学相干断层扫描和共聚焦荧光显微镜，以实现高分辨率和长WD。
A novel all-optical double gradient-index (GRIN) lens optical-resolution photoacoustic microscopy (OR-PAM), termed as DGL-PAM, is demonstrated. The miniature probe consists of a single-mode fiber and double GRIN lenses for optical focusing and a miniature fiber Fabry-Perot sensor for ultrasound detection. The new design is simple and realizes high resolution with long working distance (WD) by virtue of the double GRIN lenses. The overall size of the probe is 2.7 mm in diameter. High lateral resolution of 3.7 mu m (at 532 nm laser wavelength) and long WD of 5.5 mm are achieved. In vivo OR-PAM of mouse ear demonstrates the imaging ability of DGL-PAM. Since precise alignment of optical and acoustic foci is not needed, the proposed DGL-PAM is relatively easy to implement. It has potential to be developed as a low-cost, disposable OR-PAM probe and for endoscopic applications. The proposed double GRIN lenses for making miniature endoscopic probes can also be applied to other modalities, such as optical coherence tomography and confocal fluorescence microscopy, to enable high resolution and long WD.