L-Xylo-3-hexulose, a new rare sugar produced by the action of acetic acid bacteria on galactitol, an exception to Bertrand Hudson's rule
L-Xylo-3-hexulose, a new rare sugar produced by the action of acetic acid bacteria on galactitol, an exception to Bertrand Hudson's rule
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L-Xylo-3-hexulose，一种新的稀有糖，由乙酸细菌对半乳糖醇的作用产生，这是 Bertrand Hudson 规则的一个例外
DOI：
10.1016/j.bbagen.2020.129740
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发表时间：
2021
影响因子：
3
通讯作者：
Cheng Hairong
中科院分区：
文献类型：
--
作者：
Xu Yirong;Chi Ping;Lv Jiyang;Bilal Muhammad;Cheng Hairong
背景：在乙酸细菌中，如氧葡萄糖杆菌或葡萄球菌，吡咯喹啉。外质中的醌（PQQ）是几种膜结合脱氢酶的氧化还原辅助因子。将多水醇氧化为稀有糖，可作为传统糖和甜味剂的健康替代品。这些氧化反应遵循公认的Bertrand Hudson法则，即只有。含顺式d -红血球羟基的多羟基醇在PQQ的辅助作用下可氧化为2-酮醇，而不含顺式d -红血球羟基的多羟基醇则不能被PQQ依赖的膜结合脱氢酶氧化。方法：制备G. oxydans膜组分，并将其作为无细胞催化剂氧化半乳糖醇。结果：在本研究中，我们报道了一个有趣的氧化反应，多羟基醇半乳糖醇。（dulcitol）不具有顺式d -赤藓糖羟基，可被乙酸细菌的C-3和C-5羟基上的pqq依赖膜结合脱氢酶(s)氧化，生成稀有糖L-xylo-.3-己糖和d -塔格糖。结论：该反应可能是Bertrand Hudson法则的一个例外。一般意义：Bertrand Hudson法则是关于醋酸细菌中pqq依赖膜结合脱氢酶氧化多羟基醇的一个众所周知的理论。在这项研究中，半乳糖醇被pqq依赖的膜结合脱氢酶氧化代表了Bertrand Hudson规则的一个例外。进一步。鉴定相关酶和破译明确的酶的机制将证明这一理论。
Background: In acetic acid bacteria such as Gluconobacter oxydans or Gluconobacter cerinus, pyrroloquinoline.quinone (PQQ) in the periplasm serves as the redox cofactor for several membrane-bound dehydrogenases that.oxidize polyhydric alcohols to rare sugars, which can be used as a healthy alternative for traditional sugars and.sweeteners. These oxidation reactions obey the generally accepted Bertrand Hudson's rule, in which only the.polyhydric alcohols that possess cis D-erythro hydroxyl groups can be oxidized to 2-ketoses using PQQ as a.cofactor, while the polyhydric alcohols excluding cis D-erythro hydroxyl groups ruled out oxidation by PQQdependent membrane-bound dehydrogenases..Methods: Membrane fractions of G. oxydans were prepared and used as a cell-free catalyst to oxidize galactitol,.with or without PQQ as a cofactor..Results: In this study, we reported an interesting oxidation reaction that the polyhydric alcohols galactitol.(dulcitol), which do not possess cis D-erythro hydroxyl groups, can be oxidized by PQQ-dependent membranebound dehydrogenase(s) of acetic acid bacteria at the C-3 and C-5 hydroxyl groups to produce rare sugars L-xylo-.3-hexulose and D-tagatose..Conclusions: This reaction may represent an exception to Bertrand Hudson's rule..General significance: Bertrand Hudson's rule is a well-known theory in polyhydric alcohols oxidation by PQQdependent membrane-bound dehydrogenase in acetic acid bacteria. In this study, galactitol oxidation by a PQQdependent membrane-bound dehydrogenase represents an exception to the Bertrand Hudson's rule. Further.identification of the associated enzymes and deciphering the explicit enzymatic mechanism will prove this.theory.