Linkage mapping and physical localization of the major histocompatibility complex region of the marsupial Monodelphis domestica
Linkage mapping and physical localization of the major histocompatibility complex region of the marsupial Monodelphis domestica
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DOI：
10.1159/000089882
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发表时间：
2006-01-01
影响因子：
1.7
通讯作者：
Samollow, PB
中科院分区：
文献类型：
--
作者：
Gouin, N;Deakin, JE;Samollow, PB
我们使用遗传连锁图谱和荧光原位杂交（FISH）进行基因组织和染色体定位的主要组织相容性复合体（MHC）的有袋动物，灰色，短尾负鼠Monodelphis南极洲的第一次分析。以家系为基础的连锁分析表明，两个M。结果表明，家蚕MHC Ⅰ类基因（UA 1、UG）和3个MHC Ⅱ类基因（DAB、DMA和DMB）紧密连锁，位于第3连锁群（LG 3）的中心区域。通过使用含有UA 1基因的BAC克隆的FISH，将该MHC基因簇物理地定位到染色体2 q的着丝粒区域。一个有趣的发现，从连锁分析是，性别特异性重组率几乎是相同的MHC区域内。这与全基因组的情况形成鲜明对比，其中男性表现出约两倍的重组女性，并可能有进化的影响，保持平等的男性和女性之间的能力，在该地区产生单倍型多样性。这些分析还表明，位于人类6号染色体MHC区域侧翼的三个非MHC基因，即髓鞘少突胶质细胞糖蛋白（MOG）、骨形态发生蛋白6（BMP 6）和催乳素（PRL），在M中被分成两个独立的连锁群（染色体）。南极洲与其他8种脊椎动物的比较分析表明，尽管BMP 6- PRL -MHC-ME 1同线性不保守，但BMP 6-PRL同线性在鸟类和哺乳动物中具有很强的保守性。
We used genetic linkage mapping and fluorescence in situ hybridization ( FISH) to conduct the first analysis of genic organization and chromosome localization of the major histocompatibility complex ( MHC) of a marsupial, the gray, short-tailed opossum Monodelphis domestica. Family based linkage analyses of two M. domestica MHC Class I genes ( UA1, UG) and three MHC Class II genes ( DAB, DMA, and DMB) revealed that these genes were tightly linked and positioned in the central region of linkage group 3 ( LG3). This cluster of MHC genes was physically mapped to the centromeric region of chromosome 2q by FISH using a BAC clone containing the UA1 gene. An interesting finding from the linkage analyses is that sex-specific recombination rates were virtually identical within the MHC region. This stands in stark contrast to the genome-wide situation, wherein males exhibit approximately twice as much recombination as females, and could have evolutionary implications for maintaining equality between males and females in the ability to generate haplotype diversity in this region. These analyses also showed that three non-MHC genes that flank the MHC region on human chromosome 6, myelin oligodendrocyte glycoprotein ( MOG), bone morphogenetic protein 6 ( BMP6), and prolactin ( PRL), are split among two separate linkage groups ( chromosomes) in M. domestica. Comparative analysis with eight other vertebrate species suggests strong conservation of the BMP6-PRL synteny among birds and mammals, although the BMP6 - PRL - MHC - ME1 synteny is not conserved.