Quantification of trans-resveratrol and its metabolites in rat plasma and tissues by HPLC
Quantification of trans-resveratrol and its metabolites in rat plasma and tissues by HPLC
复制标题
DOI：
10.1016/j.jpba.2009.03.026
复制
发表时间：
2010-01-20
影响因子：
3.4
通讯作者：
Planas, Joana M.
中科院分区：
文献类型：
--
作者：
Emilia Juan, M.;Maijo, Monica;Planas, Joana M.
反式白藜芦醇是一种来自葡萄的多酚，被认为是一种对健康具有潜在有益影响的生物活性剂。然而，由于缺乏准确、精密的检测方法，人们对它在生物体内的分布知之甚少。因此，本研究的目的是开发一种通过 HPLC 提取和定量血浆、脑、睾丸、肝脏、肺和肾脏中反式白藜芦醇及其代谢物的方法。为此，根据不同的组织调整均质和液体提取的时间。该方法使用掺有纯陷阱白藜芦醇的均质组织进行了验证。精巢的精确度 (%R.S.D.) 为 3.7%，肺的精确度为 13.2%。回收率为 98.5 +/- 3.2%（肝脏）、100.1 +/- 1.8%（肾脏）、96.5 +/- 7.6%（肺）、99.0 +/- 0.7%（大脑）和 103 +/- 2.7%（睾丸）。检测限范围为睾丸中的 5.5 nM 至肾脏中的 11.2 nM。经验证，该方法用于评估白藜芦醇15 mg/kg静脉给药后大鼠体内的生物利用度和分布。 90 分钟时，陷阱-白藜芦醇及其葡萄糖醛酸和硫酸盐结合物广泛分布在所有研究的组织中。肾脏中浓度最高（nmol/g 组织）（白藜芦醇：1.45 +/- 0.35；葡萄糖醛酸苷：2.91 +/- 0.19：硫酸盐：未检测到），脑中浓度最低（白藜芦醇：0.17 +/- 0.04；葡萄糖醛酸苷：未检测到；硫酸盐：0.04 +/- 0.01）。总之，已经描述了准确且可重复的方法来识别白藜芦醇的靶组织，这是了解其体内作用机制的第一步。 (C) 2009 Elsevier B.V. 保留所有权利。
trans-Resveratrol, a polyphenol from grapes, is being recognized as a bioactive agent with potential beneficial effects on health. However, little is known about its distribution in the organism mainly because of the lack of accurate and precise detection methods. Consequently the aim of the present study was to develop a methodology of extraction and quantification of trans-resveratrol and its metabolites in plasma, brain, testis, liver, lungs and kidney by HPLC. To this end, the time of homogenization and liquid extraction were adapted to the different tissues. The methods were validated using homogenized tissues spiked with pure traps-resveratrol. The precision (%R.S.D.) ranged from 3.7% in testis to 13.2% in lungs. Recoveries were 98.5 +/- 3.2% (liver), 100.1 +/- 1.8% (kidney), 96.5 +/- 7.6% (lungs), 99.0 +/- 0.7% (brain) and 103 +/- 2.7% (testicle). The limits of detection ranged from 5.5 nM in testis to 11.2 nM in kidney. After validation, the methods were applied to the assessment of the bioavailability and distribution of traps-resveratrol in rats after the intravenous administration of 15 mg/kg. At 90 min, traps-resveratrol and its glucuronide and sulfate conjugates were widely distributed in all the tissues studied. The highest concentrations (nmol/g tissue) were found in kidney (resveratrol: 1.45 +/- 0.35; glucuronide: 2.91 +/- 0.19: sulfate: not detected), and the lowest in brain (resveratrol: 0.17 +/- 0.04; glucuronide: not detected; sulfate: 0.04 +/- 0.01). In conclusion, accurate and reproducible methods have been described to identify target tissues of resveratrol as a first step to understand its mechanisms of action in vivo. (C) 2009 Elsevier B.V. All rights reserved.