Peripheral nerve allografts stored in green tea polyphenol solution
Peripheral nerve allografts stored in green tea polyphenol solution
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DOI：
10.1097/01.tp.0000155417.87823.17
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发表时间：
2005-03-27
期刊：
影响因子：
6.2
通讯作者：
Nakamura, T
中科院分区：
文献类型：
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作者：
Ikeguchi, R;Kakinoki, R;Nakamura, T
背景我们以前证明了周围神经节段在绿色茶多酚提取物中成功储存1个月。我们研究了这种方法是否可以减少周围神经移植相关的供体-宿主免疫反应。从黑琼脂（DA）大鼠收获坐骨神经节段（20 mm长），在多酚溶液（1 mg/mL）中储存1个月，并移植到受体主要组织相容性复合物-不匹配的刘易斯大鼠中以桥接15 mm长的坐骨神经间隙（多酚处理的同种异体移植物组）。对照组为同系移植组（将从刘易斯大鼠获得的神经节段立即移植到刘易斯大鼠中）、多酚处理的同系移植组（将从刘易斯大鼠获得的神经节段以相同的方法用多酚处理并移植到刘易斯大鼠中）和新鲜同种异体移植组（将从DA大鼠获得的神经节段移植到刘易斯大鼠中而不储存）。为了研究移植神经中细胞的来源，将雄性DA大鼠供体的坐骨神经节段移植到雌性刘易斯大鼠受体中;从每个神经节段中提取基因组DNA，并使用大鼠Y染色体性别决定区（Sry）特异性引物进行聚合酶链反应扩增。同种异体神经移植组神经再生情况与同种异体神经移植组相似。Sry特异性条带在性别不匹配的多酚处理的同种异体移植标本中的所有样本中检测到，尽管它们的主要组织相容性复合体不相容。结论.保存在绿色茶多酚溶液中可以减少同种移植后神经组织的缺血性损伤和供宿主免疫反应。
Background. We previously demonstrated the successful 1 -month storage of peripheral nerve segments in a green tea polyphenol extract. We investigated whether this method could reduce the donor-host immune reaction associated with peripheral nerve allotransplantation.Methods. Sciatic nerve segments (20 mm long) were harvested from Dark Agouti (DA) rats, stored in polyphenol solution (1 mg/mL) for I month, and transplanted into recipient major histocompatibility complex- mismatched Lewis rats to bridge 15-mm-long sciatic nerve gaps (polyphenol-treated allograft group). The controls were an isograft group (nerve segments harvested from Lewis rats were immediately transplanted into Lewis rats), a polyphenol-treated isograft group (nerve segments harvested from Lewis rats were treated by polyphenol in the same method and transplanted into Lewis rats), and a fresh allograft group (nerve segments harvested from DA rats were transplanted into Lewis rats without storage). To investigate the origins of the cells in the transplanted nerves, sciatic nerve segments harvested from the male DA rat donors were transplanted into female Lewis rat recipients; genomic DNA was extracted from each nerve segment and amplified by polymerase chain reaction using primers specific for the rat sex-determining region of the Y-chromosome (Sry).Results. Nerve regeneration in the polyphenol-treated allograft group was similar to that in the isografted group. Sry-specific bands were detected in all samples in the sex-mismatched polyphenol-treated allograft specimens despite their major histocompatibility complex incompatibility. Conclusions. Storage in green tea polyphenol solution can reduce both ischemic damage to nerve tissue and donor-host immune reactions after allotransplantation.