Molecular cloning of silkworm paralytic peptide and its developmental regulation
Molecular cloning of silkworm paralytic peptide and its developmental regulation
复制标题
DOI：
10.1006/bbrc.2001.5365
复制
发表时间：
2001-08-10
影响因子：
3.1
通讯作者：
Kiuchi, M
中科院分区：
文献类型：
--
作者：
Kamimura, M;Nakahara, Y;Kiuchi, M
家蚕麻痹肽（PP）是ENF肽家族的成员，具有多种生物学活性，参与防御反应和生长调节。我们分离其cDNA并检测mRNA表达谱。cDNA编码131个氨基酸，其中23个残基的PP序列被发现在C-末端部分。免疫印迹分析和麻痹活性测定表明，幼虫血淋巴中无活性的前蛋白在出血后立即被加工成活性肽。在末龄幼虫中，0.6kb的PP mRNA在各种组织中表达，其中脂肪体占主导地位。其在脂肪体中的表达随摄食期的增加而降低，随变质期的增加而增加。保幼激素和20-羟基蜕皮激素上调其表达。在胚胎期，除了0.6kb的mRNA，1.5kb的mRNA表达，从产卵后1天孵化。因此，PP表达阶段特异性激素控制下。(C)北京：科学出版社.
The silkworm paralytic peptide (PP) is a member of the ENF peptide family that exerts multiple biological activities involved in defense reaction and growth regulation. We isolated its cDNA and examined mRNA expression profiles. cDNA encoded 131 amino acids from which the 23-residue PP sequence was found at the C-terminal portion. Immunoblot analysis and paralytic activity assay indicated that inactive proprotein in larval hemolymph was processed into active peptide immediately after bleeding. In the last larval instar, 0.6-kb PP mRNA was expressed in various tissues, of which the fat body was predominant. Its expression in the fat body decreased during the feeding period and then increased during metamorphic process. Juvenile hormone and 20-hydroxyecdysone upregulated its expression. At the embryonic stage, 1.5-kb mRNA, in addition to 0.6-kb mRNA, was expressed from 1 day after oviposition to hatching. PP was thus expressed stage-specifically under hormonal control. (C) 2001 Academic Press.