Complementary analyses of the transcriptome and iTRAQ proteome revealed mechanism of ethylene dependent salt response in bread wheat (Triticum aestivum L.)
Complementary analyses of the transcriptome and iTRAQ proteome revealed mechanism of ethylene dependent salt response in bread wheat (Triticum aestivum L.)
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转录组和 iTRAQ 蛋白质组的互补分析揭示了面包小麦 (Triticum aestivum L.) 乙烯依赖性盐反应的机制
DOI：
10.1016/j.foodchem.2020.126866
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发表时间：
2020-09-30
期刊：
影响因子：
8.8
通讯作者：
Liu, Yiguo
中科院分区：
文献类型：
--
作者：
Ma, Qian;Shi, Changhai;Liu, Yiguo
为了阐明小麦乙烯依赖性盐胁迫的响应机制，以小麦品种'青麦6号' 2周龄幼苗为材料，分别用水、NaCl、NaCl和乙烯前体1-氨基环丙烷-1-羧酸（ACC）处理，和NaCl和乙烯信号传导抑制剂1-甲基环丙烯（1-MCP），并通过转录测序和同量异序标签进行相对和绝对定量分析（iTRAQ）蛋白质组学。至少鉴定了1140种蛋白质和73，401种基因，并且蛋白质包括核糖体蛋白（RP）、核苷二磷酸激酶（CDPKs）、转醛醇酶（TAL）、β-葡萄糖醛酸酶（BGLU）、磷酸烯醇丙酮酸羧化酶（PEPC）、超氧化物歧化酶（SOD）和6-磷酸葡萄糖酸脱氢酶（6-PGDH）的表达显著不同。这些基因和蛋白揭示了乙烯依赖的盐响应通过RPs激活、分子伴侣合成、活性氧（ROS）清除和碳水化合物代谢途径。本研究结果为乙烯调控盐胁迫反应的分子机制提供了转录组学和蛋白质组学信息。
In order to clarify the ethylene dependent salt response mechanism in wheat, 2-week-old wheat seedlings of cultivar 'Qingmai 6' treated with water, sodium chloride (NaCl), NaCl and ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC), and NaCl and ethylene signaling inhibitor 1-methylcyclopropene (1-MCP) were collected and analyzed by transcriptional sequencing and isobaric tags for relative and absolute quantitation (iTRAQ) proteomics. At least 1140 proteins and 73,401 genes were identified, and proteins including ribosomal proteins (RPs), nucleoside diphosphate kinases (CDPKs), transaldolases (TALs), beta-glucosidases (BGLUs), phosphoenlpyruvate carboxylases (PEPCs), superoxide dismutases (SODs), and 6-phosphogluconate dehydrogenases (6-PGDHs) were significantly differently expressed. These genes and proteins revealed that ethylene dependent salt response through RPs activation, chaperones synthesis, the reactive oxygen species (ROS) scavenging, and carbohydrate metabolites pathway. Our results provided transcriptomics and proteomics information with respect to the molecular mechanisms of ethylene regualted salt response.