The Phage-Encoded N-Acetyltransferase Rac Mediates Inactivation of Pseudomonas aeruginosa Transcription by Cleavage of the RNA Polymerase Alpha Subunit.
The Phage-Encoded N-Acetyltransferase Rac Mediates Inactivation of Pseudomonas aeruginosa Transcription by Cleavage of the RNA Polymerase Alpha Subunit.
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DOI：
10.3390/v12090976
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发表时间：
2020-09-02
期刊：
影响因子：
--
通讯作者：
Lavigne R
中科院分区：
文献类型：
--
作者：
Ceyssens PJ;De Smet J;Wagemans J;Akulenko N;Klimuk E;Hedge S;Voet M;Hendrix H;Paeshuyse J;Landuyt B;Xu H;Blanchard J;Severinov K;Lavigne R
在这项研究中，我们描述了噬菌体编码的蛋白质RNA聚合酶α亚基裂解蛋白（Rac）的生物学功能，预测GCN 5相关的乙酰转移酶编码的phiKMV样病毒。这些细菌编码单亚基RNA聚合酶，用于转录其晚期（结构和裂解相关）基因，而细菌RNA聚合酶用于感染的早期阶段。Rac通过在细菌RNA聚合酶的α亚基中引入特异性切割来介导细菌转录的失活。这种切割发生在柔性接头序列内，并断开C-末端结构域，这是从大多数高活性细胞启动子转录起始所需的。为了实现这一目标，Rac可能会在宿主铜绿假单胞菌中引入一种新的翻译后修饰（PTM）机制。从进化的角度来看，这种新的噬菌体编码的调节机制证实了PTM在原核代谢中的重要性，并代表了一种新的方式，通过它可以劫持细菌宿主代谢。
In this study, we describe the biological function of the phage-encoded protein RNA polymerase alpha subunit cleavage protein (Rac), a predicted Gcn5-related acetyltransferase encoded by phiKMV-like viruses. These phages encode a single-subunit RNA polymerase for transcription of their late (structure- and lysis-associated) genes, whereas the bacterial RNA polymerase is used at the earlier stages of infection. Rac mediates the inactivation of bacterial transcription by introducing a specific cleavage in the α subunit of the bacterial RNA polymerase. This cleavage occurs within the flexible linker sequence and disconnects the C-terminal domain, required for transcription initiation from most highly active cellular promoters. To achieve this, Rac likely taps into a novel post-translational modification (PTM) mechanism within the host Pseudomonas aeruginosa. From an evolutionary perspective, this novel phage-encoded regulation mechanism confirms the importance of PTMs in the prokaryotic metabolism and represents a new way by which phages can hijack the bacterial host metabolism.
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