Complete genome sequence of an aerobic hyper-thermophilic crenarchaeon, Aeropyrum pernix K1.
Complete genome sequence of an aerobic hyper-thermophilic crenarchaeon, Aeropyrum pernix K1.
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DOI：
10.1093/dnares/6.2.83
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发表时间：
1999-04-30
期刊：
影响因子：
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通讯作者：
Kikuchi, H
中科院分区：
文献类型：
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作者：
Kawarabayasi, Y;Hino, Y;Kikuchi, H
一个好氧的超嗜热crenarcheon，Aeropyrum pernix K1，最佳生长在95摄氏度的基因组的完整序列，已确定的全基因组鸟枪法与一些修改。基因组全长为1，669，695 bp。整个序列的真实性得到了长PCR产物的限制性分析的支持，这些产物直接从基因组DNA扩增。作为潜在的蛋白质编码区，共分配了2，694个开放阅读框（ORF）。通过与公共数据库的相似性检索，633个（23.5%）与已知功能的基因相关，523个（19.4%）与已注册但功能未知的基因相关。包括TCA循环中除α-酮戊二酸脱氢酶以外的所有基因，并且鉴定了编码2-含氧酸的两个亚基的基因：铁氧还蛋白氧化还原酶，而不是α-酮戊二酸脱氢酶基因。其余1，538个ORF（57.1%）与数据库中的序列没有任何显著相似性。序列比较表明，相当一部分ORF是由序列重复产生的。所鉴定的RNA基因包括1个16 S-23 S rRNA操纵子、2个5S rRNA基因和47个tRNA基因，其中14个基因具有内含子结构。所有的ORF和RNA编码区占全基因组的89.12%。本文提供的数据可在互联网主页（http：//www.example.com）上查阅。www.mild.nite.go.jp
The complete sequence of the genome of an aerobic hyper-thermophilic crenarchaeon, Aeropyrum pernix K1, which optimally grows at 95 degrees C, has been determined by the whole genome shotgun method with some modifications. The entire length of the genome was 1,669,695 bp. The authenticity of the entire sequence was supported by restriction analysis of long PCR products, which were directly amplified from the genomic DNA. As the potential protein-coding regions, a total of 2,694 open reading frames (ORFs) were assigned. By similarity search against public databases, 633 (23.5%) of the ORFs were related to genes with putative function and 523 (19.4%) to the sequences registered but with unknown function. All the genes in the TCA cycle except for that of alpha-ketoglutarate dehydrogenase were included, and instead of the alpha-ketoglutarate dehydrogenase gene, the genes coding for the two subunits of 2-oxoacid:ferredoxin oxidoreductase were identified. The remaining 1,538 ORFs (57.1%) did not show any significant similarity to the sequences in the databases. Sequence comparison among the assigned ORFs suggested that a considerable member of ORFs were generated by sequence duplication. The RNA genes identified were a single 16S-23S rRNA operon, two 5S rRNA genes and 47 tRNA genes including 14 genes with intron structures. All the assigned ORFs and RNA coding regions occupied 89.12% of the whole genome. The data presented in this paper are available on the internet homepage (http://www.mild.nite.go.jp).