Molecular cloning and characterization of rKAB1, which interacts with KARP-1, localizes in the nucleus and protects cells against oxidative death
Molecular cloning and characterization of rKAB1, which interacts with KARP-1, localizes in the nucleus and protects cells against oxidative death
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DOI：
10.1023/a:1024157515342
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发表时间：
2003-06
影响因子：
4.3
通讯作者：
E. Do;E. Taira;Y. Irie;Y. Gan;Hidekazu Tanaka;C. Kuo;N. Miki
中科院分区：
文献类型：
--
作者：
E. Do;E. Taira;Y. Irie;Y. Gan;Hidekazu Tanaka;C. Kuo;N. Miki
Ku自身抗原/KARP-1（Ku 86 autoantigen related protein-1）作为DNA依赖性蛋白激酶（DNA-PK）复合物的DNA结合组分，在哺乳动物DNA双链断裂修复中发挥重要作用。KARP-1与人Ku 86自身抗原基因座不同地转录，并且其涉及DNA依赖性蛋白激酶活性的控制。我们从大鼠海马cDNA文库中克隆了人KARP-1结合蛋白1（KAB 1）的大鼠同源物rKAB 1。rKAB 1 mRNA在脑和胸腺中特异性表达。EGFP标记的rKAB 1蛋白定位于细胞核和有丝分裂时的染色体上。我们发现，rKAB 1作为一种保护蛋白，对抗氧化应激引起的细胞损伤。
The Ku autoantigen/KARP-1 (Ku86 autoantigen related protein-1) plays an important role in the double-strand break repair of mammalian DNA as a DNA-binding component of DNA-dependent protein kinase (DNA-PK) complex. KARP-1 is differently transcribed from the human Ku86 autoantigen gene locus and it is implicated in the control of DNA-dependent protein kinase activity. We cloned rKAB1, a rat homolog of KAB1 (KARP-1 binding protein 1 of human) from a rat hippocampal cDNA library. rKAB1 mRNA was specifically expressed in the brain and the thymus. EGFP-tagged rKAB1 protein localized in cell nucleus and in the condensed chromosome during the mitotic cell division. We found that rKAB1 works as a protective protein against cell damage by oxidative stress.