Tomato lycopene and low density lipoprotein oxidation: A human dietary intervention study
Tomato lycopene and low density lipoprotein oxidation: A human dietary intervention study
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DOI：
10.1007/s11745-998-0295-6
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发表时间：
1998-10-01
期刊：
影响因子：
1.9
通讯作者：
Rao, AV
中科院分区：
文献类型：
--
作者：
Agarwal, S;Rao, AV
低密度脂蛋白（LDL）氧化的增加被认为与动脉粥样硬化和冠心病的风险增加有因果关系。在最近的流行病学研究中，组织和血清中番茄红素（一种从番茄中提取的类胡萝卜素）的水平与冠心病的风险呈负相关。对19名健康人膳食补充番茄红素对LDL氧化的影响进行了研究。以番茄汁、意大利面酱和番茄油树脂分别饲喂番茄红素，各饲喂1周。在每次治疗结束时采集血液样本。采用高效液相色谱法提取血清番茄红素，并用吸光度检测器进行测定。用缓冲肝素沉淀法分离血清LDL，测定硫代巴比妥酸反应物质（TBARS）和共轭二烯（CD）来评估LDL氧化。两种检测LDL氧化的方法LDL- tbars和LDL- cd结果一致。饲粮中添加番茄红素可显著提高血清番茄红素水平至少两倍。虽然血清胆固醇水平（总胆固醇、低密度脂蛋白或高密度脂蛋白）没有变化，但血清脂质过氧化和低密度脂蛋白氧化显著降低。这些结果可能与降低冠心病的风险有关。
Increase in low density lipoprotein (LDL) oxidation is hypothesized to be causally associated with increasing risk of atherosclerosis and coronary heart disease. In recent epidemiological studies, tissue and serum levels of lycopene, a carotenoid available from tomatoes, have been round to be inversely related to risk of coronary heart disease. A study was undertaken to investigate the effect of dietary supplementation of lycopene on LDL oxidation in 19 healthy human subjects. Dietary lycopene was provided using tomato juice, spaghetti sauce, and tomato oleoresin for a period of 1 wk each. Blood samples were collected at the end of each treatment. Serum lycopene was extracted and measured by high-performance liquid chromatography using an absorbance detector. Serum LDL was isolated by precipitation with buffered heparin, and thiobarbituric acid-reactive substances (TBARS) and conjugated dienes (CD) were measured to estimate LDL oxidation. Both methods, to measure LDL oxidation LDL-TBARS and LDL-CD, were in good agreement with each other. Dietary supplementation of lycopene significantly increased serum lycopene levels by at least twofold. Although there was no change in serum cholesterol levels (total, LDL, or high-density lipoprotein), serum lipid peroxidation and LDL oxidation were significantly decreased. These results may have relevance for decreasing the risk for coronary heart disease.