An alternative to the accepted phylogeny of purple bacteria based on 16S rRNA:: Analyses of the amino acid sequences of cytochromes C2 and C556 from Rhodobacter (Rhodovulum) sulfidophilus
An alternative to the accepted phylogeny of purple bacteria based on 16S rRNA:: Analyses of the amino acid sequences of cytochromes C2 and C556 from Rhodobacter (Rhodovulum) sulfidophilus
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DOI：
10.1006/abbi.2000.2221
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发表时间：
2001-04-01
影响因子：
3.9
通讯作者：
Cusanovich, MA
中科院分区：
文献类型：
--
作者：
Ambler, RP;Meyer, TE;Cusanovich, MA
从全基因组序列中越来越明显的是，目前解释的16S rRNA数据不能提供细菌同源性的明确图像。相反，我们发现细胞色素c（2）氨基酸序列中插入和缺失的分析在建立关系方面具有一些优势，并且这种方法在更好地理解细菌关系方面可能具有广泛的实用性。测定了4株嗜硫红杆菌细胞色素c（2）和c556的全部或部分氨基酸序列。细胞色素c（2）与大细胞色素c（2）一样，含有3个和8个残基的插入以及一个单残基的缺失，但与小细胞色素c（2）和线粒体细胞色素不同。此外，Rb。嗜硫菌蛋白与其他红细菌细胞色素c共享一个罕见的六到七个残基插入（2）。细胞色素c556是低自旋II类细胞色素c，与通常为高自旋的细胞色素c '的大家族同源。细胞色素c556的相似性，以其他物种的第二类细胞色素是一致的，从细胞色素c的比较推导出的关系。因此，我们的结果不支持Rb的位置。在一个单独的属Rhodovulum中发现了嗜硫菌，这主要是基于16S rRNA序列提出的。相反，红细菌的细胞色素C，是不同于其他属和种的紫色细菌，并显示出不同的模式的物种之间的关系比16S rRNA的报告。(C)北京：科学出版社.
It is becoming increasingly apparent from complete genome sequences that 16S rRNA data, as currently interpreted, does not provide an unambiguous picture of bacterial phylogeny, In contrast, we have found that analysis of insertions and deletions in the amino acid sequences of cytochrome c(2) has some advantages in establishing relationships and that this approach may have broad utility in acquiring a better understanding of bacterial relationships. The amino acid sequences of cytochromes c(2) and c556 have been determined in whole or in part from four strains of Rhodobacter sulfidophilus. The cytochrome c(2) contains three- and eight-residue insertions as well as a single-residue deletion in common with the large cytochromes c(2) but in contrast to the small cytochromes c(2) and mitochondrial cytochromes. In addition, the Rb. sulfidophilus protein shares a rare six- to seven-residue insertion with other Rhodobacter cytochromes c(2). The cytochrome c556 is a low-spin class II cytochrome c homologous to the greater family of cytochromes c', which are usually high-spin. The similarity of cytochrome c556 to other species of class II cytochromes is consistent with the relationships deduced from comparisons of cytochromes c,. Thus, our results do not support placement of Rb. sulfidophilus in a separate genus, Rhodovulum, which was proposed primarily on the basis of 16S rRNA sequences. Instead, the Rhodobacter cytochromes c, are distinct from those of other genera and species of purple bacteria and show a different pattern of relationships among species than reported for 16S rRNA. (C) 2001 Academic Press.