Molecular crowding stabilizes folded RNA structure by the excluded volume effect.
Molecular crowding stabilizes folded RNA structure by the excluded volume effect.
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DOI：
10.1021/ja101500g
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发表时间：
2010-06-30
影响因子：
15
通讯作者：
Woodson, Sarah A.
中科院分区：
文献类型：
--
作者：
Kilburn, Duncan;Roh, Joon Ho;Guo, Liang;Briber, Robert M.;Woodson, Sarah A.
溶液中的克劳德分子改变了生物大分子折叠和未折叠状态之间的平衡。因此，在描述RNA在细胞内的折叠时，考虑到这些其他分子的影响是至关重要的。报道了在平均分子质量为1000Da的聚乙二醇1000(PEG1000)存在下，kDa细菌I群核酶的小角X射线散射实验。与预期的排除体积效应一致，聚乙二醇有利于更紧密的RNA结构。首先，在聚乙二醇中较低浓度的氯化镁溶液中，发生了从未折叠到折叠(更紧密)的转变。其次，随着聚乙二醇浓度从0增加到20%重量/体积分数，未折叠核糖核酸的旋转半径从76？减小到？实验测量了水和离子活度的变化，并用理论模型估算了排除体积。我们得出结论：聚乙二醇克劳德对折叠过程的主要影响是排除体积效应。
Crowder molecules in solution alter the equilibrium between folded and unfolded states of biological macromolecules. It is therefore critical to account for the influence of these other molecules when describing the folding of RNA inside the cell. Small angle x-ray scattering experiments are reported on a 64 kDa bacterial group I ribozyme in the presence of polyethylene-glycol 1000 (PEG-1000), a molecular crowder with average molecular weight 1000 Da. In agreement with expected excluded volume effects, PEG favors more compact RNA structures. Firstly, the transition from the unfolded to the folded (more compact) state occurs at lower MgCl2 concentrations in PEG. Secondly, the radius of gyration of the unfolded RNA decreases from 76 Å to 64 Å as the PEG concentration increases from 0 to 20 % wt./vol. Changes to water and ion activities were measured experimentally, and theoretical models were used to evaluate the excluded volume. We conclude that the dominant influence of the PEG crowder on the folding process is the excluded volume effect.
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影响因子：
5.6
作者：
Heilman-Miller, SL;Thirumalai, D;Woodson, SA
通讯作者：
Woodson, SA
影响因子：
15
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Koculi, Eda;Hyeon, Changbong;Woodson, Sarah A.
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Woodson, Sarah A.
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Moghaddam, Sarvin;Caliskan, Gokhan;Chauhan, Seema;Hyeon, Changbong;Briber, R. M.;Thirumalai, D.;Woodson, Sarah A.
通讯作者：
Woodson, Sarah A.