A psychological stressor conveyed by appetite-linked neurons
A psychological stressor conveyed by appetite-linked neurons
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DOI：
10.1126/sciadv.aay5366
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发表时间：
2020-03-01
期刊：
影响因子：
13.6
通讯作者：
Buck, Linda B.
中科院分区：
文献类型：
--
作者：
Lee, Eun Jeong;Hanchate, Naresh K.;Buck, Linda B.
哺乳动物对危险表现出本能的反应，这对生存至关重要，包括血液应激激素的激增。下丘脑促肾上腺皮质激素释放激素神经元（CRHNs）控制应激激素，但不同的应激源如何汇聚CRHNs知之甚少。我们使用sRNA分析来定义神经递质和神经调质的CRHN受体，然后使用病毒示踪来定位表达同源受体配体的上游神经元的子集。出乎意料的是，一个子集包括弓状核中的POMC（前阿黑皮素）表达神经元，这与食欲抑制有关。POMC神经元被一种心理应激源激活，物理约束，而不是另一种，捕食者气味。化学发生激活POMC神经元诱导应激激素反应，模仿应激源。此外，他们的沉默显着减少了应激激素对物理约束的反应，但不是捕食者的气味。这些发现表明，参与食欲抑制的POMC神经元也在应激激素对特定类型的心理应激源的反应中发挥重要作用。
Mammals exhibit instinctive reactions to danger critical to survival, including surges in blood stress hormones. Hypothalamic corticotropin-releasing hormone neurons (CRHNs) control stress hormones but how diverse stressors converge on CRHNs is poorly understood. We used sRNA profiling to define CRHN receptors for neurotransmitters and neuromodulators and then viral tracing to localize subsets of upstream neurons expressing cognate receptor ligands. Unexpectedly, one subset comprised POMC (proopiomelanocortin)-expressing neurons in the arcuate nucleus, which are linked to appetite suppression. The POMC neurons were activated by one psychological stressor, physical restraint, but not another, a predator odor. Chemogenetic activation of POMC neurons induced a stress hormone response, mimicking a stressor. Moreover, their silencing markedly reduced the stress hormone response to physical restraint, but not predator odor. These findings indicate that POMC neurons involved in appetite suppression also play a major role in the stress hormone response to a specific type of psychological stressor.