Feasibility of using multiphoton excited tissue autofluorescence for in vivo human histopathology.
Feasibility of using multiphoton excited tissue autofluorescence for in vivo human histopathology.
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DOI：
10.1364/boe.1.001320
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发表时间：
2010-11-05
影响因子：
3.4
通讯作者：
Zipfel WR
中科院分区：
文献类型：
--
作者：
Dela Cruz JM;McMullen JD;Williams RM;Zipfel WR
显微组织形态学和病理学的快速和直接成像可以通过内在组织荧光团和二次谐波信号的多光子成像来实现。开发用于临床应用的该技术的工程参数包括从不同组织类型获得诊断质量图像所需的激发水平和收集效率，以及这些水平是否具有致突变性。在这里，我们提供了典型的平均功率所需的高信噪比在体内组织成像的数据，并评估这些辐照度水平的潜在风险，使用哺乳动物细胞基因突变检测。每个细胞暴露于760 nm、约200 fs光栅扫描激光照射的时间约为16毫秒，通过0.75 NA物镜传递，在功率高达约50 mW时产生的致突变性可忽略不计。
Rapid and direct imaging of microscopic tissue morphology and pathology can be achieved by multiphoton imaging of intrinsic tissue fluorophores and second harmonic signals. Engineering parameters for developing this technology for clinical applications include excitation levels and collection efficiencies required to obtain diagnostic quality images from different tissue types and whether these levels are mutagenic. Here we provide data on typical average powers required for high signal-to-noise in vivo tissue imaging and assess the risk potential of these irradiance levels using a mammalian cell gene mutation assay. Exposure times of ~16 milliseconds per cell to 760 nm, ~200 fs raster-scanned laser irradiation delivered through a 0.75 NA objective produced negligible mutagenicity at powers up to about 50 mW.