Bmc Molecular Biology Characterization of Housekeeping Genes in Zebrafish: Male-female Differences and Effects of Tissue Type, Developmental Stage and Chemical Treatment
Bmc Molecular Biology Characterization of Housekeeping Genes in Zebrafish: Male-female Differences and Effects of Tissue Type, Developmental Stage and Chemical Treatment
复制标题
DOI：
--
复制
发表时间：
--
期刊：
影响因子：
--
通讯作者：
Amy T McCurley;G. Callard;Email Amy;V. Callard
中科院分区：
文献类型：
--
作者：
Amy T McCurley;G. Callard;Email Amy;V. Callard
背景：利用斑马鱼模型的研究近年来发展迅速。虽然实时反转录PCR （QPCR）归一化为内参（“内参”）基因是一种常用的定量测定斑马鱼基因表达变化的方法，但已知许多常用的内参基因会随着实验条件的变化而变化。为了确定在不同实验条件下稳定表达的内护基因，从而适合作为斑马鱼QPCR的归一化基因，本研究评估了8种常用内护基因的表达，这些基因在发育过程中与阶段和激素/毒物暴露有关，并与成年鱼的组织类型和性别有关。
Background: Research using the zebrafish model has experienced a rapid growth in recent years. Although real-time reverse transcription PCR (QPCR), normalized to an internal reference ("housekeeping") gene, is a frequently used method for quantifying gene expression changes in zebrafish, many commonly used housekeeping genes are known to vary with experimental conditions. To identify housekeeping genes that are stably expressed under different experimental conditions, and thus suitable as normalizers for QPCR in zebrafish, the present study evaluated the expression of eight commonly used housekeeping genes as a function of stage and hormone/ toxicant exposure during development, and by tissue type and sex in adult fish.