Gene structure and alternative splicing of the mouse glycine transporter type-2
Gene structure and alternative splicing of the mouse glycine transporter type-2
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DOI：
10.1016/j.bbrc.2004.03.125
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发表时间：
2004-05-07
影响因子：
3.1
通讯作者：
Yanagawa, Y
中科院分区：
文献类型：
--
作者：
Ebihara, S;Yamamoto, T;Yanagawa, Y
2型甘氨酸转运蛋白GLYT 2在甘氨酸能神经元中表达，并参与终止士的宁敏感的甘氨酸能突触的抑制性神经传递。我们从小鼠脑中分离了GLYT 2亚型GLYT 2a的cDNA，发现它含有798个氨基酸的编码序列。我们还分离并鉴定了小鼠GLYT 2（mGLYT 2）基因，发现该基因分为18个外显子，分布在55 kb以上。cDNA末端的5 '-快速扩增分析表明，除了mGLYT 2a之外，还存在另外两种异构体mGLYT 2b和mGLYT 2c。mGLYT 2b和mGLYT 2c都会产生比mGLYT 2a短8个氨基酸的蛋白质。对包含5 '外显子的基因组克隆的分析表明，这三种转录物通过选择性剪接来自单个基因。RT-PCR分析表明，所有三种mGLYT 2亚型在脑干和脊髓中均以高水平表达。这些数据将有助于通过基因打靶实验研究GLYT 2蛋白和甘氨酸能神经元的功能。(C)2004年爱思唯尔公司All rights reserved.
The type-2 glycine transporter GLYT2 is expressed in glycinergic neurons and is involved in the termination of inhibitory neurotransmission at strychnine-sensitive glycinergic synapses. We isolated cDNA of a GLYT2 isoform, GLYT2a, from mouse brain, and found that it contains a coding sequence of 798 amino acids. We also isolated and characterized the mouse GLYT2 (mGLYT2) gene, which was found to be divided into 18 exons and spread over 55 kb. 5'-rapid amplification of cDNA ends analyses demonstrated the existence of another two isoforms, mGLYT2b and mGLYT2c, in addition to mGLYT2a. Both mGLYT2b and mGLYT2c would produce a protein eight amino acids shorter than mGLYT2a. Analysis of the genomic clones encompassing the 5'-exons revealed that the three transcripts arose from a single gene by alternative splicing. RT-PCR analysis indicated that all three mGLYT2 isoforms were expressed at high levels in brain stem and spinal cord. These data will be useful for investigating the function of GLYT2 proteins and glycinergic neurons by gene targeting experiments. (C) 2004 Elsevier Inc. All rights reserved.