Process of cellular division in Escherichia coli growth pattern of E. coli murein.
Process of cellular division in Escherichia coli growth pattern of E. coli murein.
复制标题
大肠杆菌细胞分裂过程的大肠杆菌生长模式。
DOI：
10.1016/0022-2836(73)90437-3
复制
发表时间：
1973
影响因子：
5.6
通讯作者：
U. Schwarz
中科院分区：
文献类型：
--
作者：
A. Ryter;Y. Hirota;U. Schwarz
本文用高分辨放射自显影和电子显微镜研究了决定大肠杆菌形状的胞壁素囊的生长方式。以~ 3 H标记的二氨基庚二酸作为特异性的胞壁素前体，脉冲标记胞壁素，并立即制备囊泡。放射性的新生murein出现在自动射线照片在球囊的中心区域的一个明确的生长区。无论细胞大小，这都是正确的。脉冲追踪实验显示标记的胞壁素与预先存在的胞壁素快速混合，并且其均匀分布在球囊的整个表面上。
The growth pattern of the murein-sacculus which determines the shape of theEscherichia colicell was studied by the use of high-resolution autoradiography with the electron microscope. The murein was pulse labelled with3H-labelled diaminopimelic acid as a specific murein precursor and sacculi were prepared immediately. The radioactivity of the nascent murein appeared on the auto- radiographs at a well-defined growth zone in the central area of the sacculus. This was true regardless of the size of the cells. Pulse chase experimenta show rapid mixing of labelled murein with pre-existing murein and its even distribution over the whole surface of the sacculus.