Rainfall increases the abundance of antibiotic resistance genes within a riverine microbial community
Rainfall increases the abundance of antibiotic resistance genes within a riverine microbial community
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DOI：
10.1016/j.envpol.2017.04.036
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发表时间：
2017-07-01
影响因子：
8.9
通讯作者：
Corno, Gianluca
中科院分区：
文献类型：
--
作者：
Di Cesare, Andrea;Eckert, Ester M.;Corno, Gianluca
耐抗生素细菌感染是人类健康的主要威胁之一。阐明环境在其传播中的作用的研究仍处于起步阶段，然而，似乎不同的环境可能作为驻留在其微生物群落中的抗生素抗性基因（ARG）的长期储存库。越来越多的研究针对接触人类活动的沃茨和土壤中的精氨酸和精氨酸的存在和持久性;然而，它们很少考虑这些环境中占主导地位的空间/时间变异性。在这里，我们评估了一个中雨事件（4毫米雨小时（-1））的ARG（tetA，ermB，bla（CTXM），sull，和qnrS）的丰度和分布的影响，通过比较降雨期间的基因丰度的测量值的年平均值，在一个大型亚高山河流的沃茨。在降雨过程中，ARG丰度都有所增加，然后将其与几个微生物、物理和化学变量相关联，以确定其潜在来源。降雨过程中ARG丰度的增加（总ARG：24倍）是伴随着总磷，N-NH 4，和微生物聚集体的增加。我们的研究结果表明，一个中等降雨量的ARGs丰度的强烈影响，并建议集水区作为其来源。然后，在模拟和管理ARG动态时，应考虑到人类活动暴露地区的中度干旱的影响。(C)2017爱思唯尔有限公司版权所有
Infections with antibiotic resistant bacteria are among the major threats for human health. Studies elucidating the role of the environment in their spread are still in their infancy, it, however, seems that different environments might function as a long-term reservoir of antibiotic resistance genes (ARGs) that reside within their microbial communities. An increasing number of studies target the presence and the persistence of ARGs in waters and soils that are exposed to human activities; they, however, rarely consider the spatial/temporal variability that predominate in these environments. Here we evaluated the effect of a moderate rain event (4 mm rain h(-1)) on the abundance and distribution of ARGs (tetA, ermB, bla(CTXM), sulll, and qnrS), by comparing measurements of gene abundances during the rainfall to the yearly average, in the waters of a large subalpine river. ARG abundances, which all increased during the rain event, were then correlated to several microbiological, physical and chemical variables, in order to establish their potential origin. Increments in ARG abundances during rainfall (total ARGs: 24 fold) was concomitant to an increase in total phosphorous, N-NH4, and microbial aggregates. Our results show a strong influence of a moderate rainfall on the abundances of ARGs, and suggest the catchment as their source. The impact of moderate rainfalls in areas exposed to anthropic activities should then be considered in modelling and management of ARG dynamics. (C) 2017 Elsevier Ltd. All rights reserved.