Towards analytically useful two-dimensional Fourier transform ion cyclotron resonance mass spectrometry
Towards analytically useful two-dimensional Fourier transform ion cyclotron resonance mass spectrometry
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DOI：
10.1007/s00216-012-6422-8
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发表时间：
2013-01-01
影响因子：
4.3
通讯作者：
Rolando, Christian
中科院分区：
文献类型：
--
作者：
van Agthoven, Maria A.;Delsuc, Marc-Andre;Rolando, Christian
傅里叶变换离子回旋共振（FT-ICR）质谱（MS）实现高分辨率和质量精度，允许识别复杂样品中离子的原始化学配方。利用离子分离和碎片化（MS/MS）技术，我们可以获得更多的结构信息，但由于每个离子都必须在碎片化之前被分离，因此MS/MS耗时且样品消耗大。1987年，Pfandler等人提出了二维FT-ICR质谱的实验，目的是在不分离离子的情况下对离子进行片段化，并在单一二维质谱中可视化复杂样品的片段，如二维核磁共振波谱。由于电子和计算机的限制，很少有研究使用这种技术。现代计算机的改进和FT-ICR硬件的数字电子技术的使用现在使得在很宽的质量范围内获得二维质谱成为可能。最初的实验使用细胞内碰撞诱导解离，这导致分辨率的损失。无气体破碎模式，如红外多光子离解和电子捕获离解允许一个测量高分辨率的二维质谱。因此，人们对将二维FT-ICR质谱发展成一种有效的分析方法重新产生了兴趣。我们描述了2D FT-ICR MS的历史，介绍了最近的改进，并介绍了用于绘制肽片段的分析应用。最后，我们提供了一个术语表，其中定义了2D FT-ICR MS领域的几个关键词。
Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) achieves high resolution and mass accuracy, allowing the identification of the raw chemical formulae of ions in complex samples. Using ion isolation and fragmentation (MS/MS), we can obtain more structural information, but MS/MS is time- and sample-consuming because each ion must be isolated before fragmentation. In 1987, Pfandler et al. proposed an experiment for 2D FT-ICR MS in order to fragment ions without isolating them and to visualize the fragmentations of complex samples in a single 2D mass spectrum, like 2D NMR spectroscopy. Because of limitations of electronics and computers, few studies have been conducted with this technique. The improvement of modern computers and the use of digital electronics for FT-ICR hardware now make it possible to acquire 2D mass spectra over a broad mass range. The original experiments used in-cell collision-induced dissociation, which caused a loss of resolution. Gas-free fragmentation modes such as infrared multiphoton dissociation and electron capture dissociation allow one to measure high-resolution 2D mass spectra. Consequently, there is renewed interest to develop 2D FT-ICR MS into an efficient analytical method. Improvements introduced in 2D NMR spectroscopy can also be transposed to 2D FT-ICR MS. We describe the history of 2D FT-ICR MS, introduce recent improvements, and present analytical applications to map the fragmentation of peptides. Finally, we provide a glossary which defines a few keywords for the 2D FT-ICR MS field.