Aubergine iCLIP Reveals piRNA-Dependent Decay of mRNAs Involved in Germ Cell Development in the Early Embryo.
Aubergine iCLIP Reveals piRNA-Dependent Decay of mRNAs Involved in Germ Cell Development in the Early Embryo.
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DOI：
10.1016/j.celrep.2015.07.030
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发表时间：
2015-08-18
期刊：
影响因子：
8.8
通讯作者：
Simonelig M
中科院分区：
文献类型：
--
作者：
Barckmann B;Pierson S;Dufourt J;Papin C;Armenise C;Port F;Grentzinger T;Chambeyron S;Baronian G;Desvignes JP;Curk T;Simonelig M
Piwi 相互作用 RNA (piRNA) 途径在种系转座子的抑制中发挥重要作用。 piRNA 的其他功能，例如 mRNA 的转录后调节，现在正在出现。在这里，我们使用 PIWI 蛋白茄子 (Aub) 进行 iCLIP，并鉴定了早期果蝇胚胎中与 Aub 相互作用的数百个母体 mRNA。基因表达谱揭示了这些 mRNA 的一部分在母体到合子的转变过程中经历了 Aub 依赖性的不稳定。引人注目的是，Aub 依赖性不稳定 mRNA 编码生殖细胞决定簇。具有无法结合 piRNA 的 Aub 突变体的 iCLIP 证实了 Aub 与 mRNA 的 piRNA 依赖性结合。 piRNA 和 mRNA 之间的碱基配对可以诱导 mRNA 裂解和衰变，这对于胚胎发育至关重要。这些结果表明 Aub 和 piRNA 对母体 mRNA 进行一般调节，Aub 和 piRNA 通过编码生殖细胞决定簇的 mRNA 的衰变和定位在胚胎发育中发挥关键的作用。 Aub 与早期果蝇胚胎中的母体 mRNA 结合 Aub 和母体 mRNA 之间的相互作用取决于 piRNA 在 MZT 期间，Aub 突变体在 mRNA 衰减方面存在缺陷 Aub 依赖的不稳定 mRNA 编码生殖细胞决定簇 使用 iCLIP，Barckmann 等人。鉴定出数百个与早期胚胎中 Aub 相互作用的母体 mRNA。许多这些 mRNA 在母体到合子的转变过程中在体细胞中经历 Aub 依赖性不稳定，并编码生殖细胞决定簇。
The Piwi-interacting RNA (piRNA) pathway plays an essential role in the repression of transposons in the germline. Other functions of piRNAs such as post-transcriptional regulation of mRNAs are now emerging. Here, we perform iCLIP with the PIWI protein Aubergine (Aub) and identify hundreds of maternal mRNAs interacting with Aub in the early Drosophila embryo. Gene expression profiling reveals that a proportion of these mRNAs undergo Aub-dependent destabilization during the maternal-to-zygotic transition. Strikingly, Aub-dependent unstable mRNAs encode germ cell determinants. iCLIP with an Aub mutant that is unable to bind piRNAs confirms piRNA-dependent binding of Aub to mRNAs. Base pairing between piRNAs and mRNAs can induce mRNA cleavage and decay that are essential for embryonic development. These results suggest general regulation of maternal mRNAs by Aub and piRNAs, which plays a key developmental role in the embryo through decay and localization of mRNAs encoding germ cell determinants. Aub binds to maternal mRNAs in early Drosophila embryos Interaction between Aub and maternal mRNAs depends on piRNAs aub mutants are defective in mRNA decay during the MZT Aub-dependent unstable mRNAs encode germ cell determinants Using iCLIP, Barckmann et al. identify several hundred maternal mRNAs that interact with Aub in early embryos. A number of these mRNAs undergo Aub-dependent destabilization in the soma during the maternal-to-zygotic transition and encode germ cell determinants.