p53, proliferating cell nuclear antigen, and Ki-67 expression in extrauterine leiomyosarcomas.
p53, proliferating cell nuclear antigen, and Ki-67 expression in extrauterine leiomyosarcomas.
复制标题
p53、增殖细胞核抗原和 Ki-67 在宫外平滑肌肉瘤中的表达。
作者：
P. E. O'Reilly;S. Raab;T. Niemann;J. Rodgers;R. Robinson
子宫外平滑肌瘤，因为其相对罕见，是知之甚少，关于其恶性潜能和生物学标志物。许多人类肿瘤的特征在于肿瘤抑制蛋白p53的过表达，以及与增殖相关的核蛋白如增殖细胞核抗原（PCNA）和Ki-67的表达。我们检测了44例子宫外平滑肌瘤中这些标记物的表达，这些肿瘤来自10名男性和25名女性，年龄从25岁到82岁不等（平均56岁）。35例患者中有34例（97%）获得了临床随访，用两种单克隆抗体（1801，D 07）通过抗原修复法研究了p53表达。44例中p53过表达19例（43%），Ki-67和PCNA阳性分别为29例（66%）和36例（82%）。p53、PCNA和Ki-67的表达与总生存率和复发率无相关性。我们的结论是，p53，Ki-67，PCNA表达在大量的子宫外平滑肌瘤。在这项研究中，这些标志物的表达并不能预测生物学行为。
Extrauterine leiomyosarcomas, because of their relative rarity, are poorly understood in regards to their malignant potential and biologic markers. Many human tumors are characterized by overexpression of p53, a tumor suppressor protein, and by expression of nuclear proteins associated with proliferation, such as proliferating cell nuclear antigen (PCNA) and Ki-67. We examined expression of these markers in 44 extrauterine leiomyosarcomas from 10 men and 25 women who ranged in age from 25 to 82 years (mean, 56 yr). Clinical follow-up was obtained in 34 (97%) of 35 patients, p53 expression was studied with two monoclonal antibodies (1801, D07) by use of an antigen retrieval method. p53 overexpression was present in 19 (43%) of 44 cases, whereas Ki-67 and PCNA staining were seen in 29 (66%) and 36 (82%) of 44 cases, respectively. There was no correlation between overall survival or recurrence and p53 or PCNA and Ki-67 expression. We conclude that p53, Ki-67, and PCNA are expressed in a large number of extrauterine leiomyosarcomas. In this study, the expression of these markers did not predict biologic behavior.