Dynamics of Dual Infection with Campylobacter jejuni Strains in Chickens Reveals Distinct Strain-to-Strain Variation in Infection Ecology
Dynamics of Dual Infection with Campylobacter jejuni Strains in Chickens Reveals Distinct Strain-to-Strain Variation in Infection Ecology
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DOI：
10.1128/aem.01901-14
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发表时间：
2014-10-01
影响因子：
4.4
通讯作者：
Williams, Nicola
中科院分区：
文献类型：
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作者：
Chaloner, Gemma;Wigley, Paul;Williams, Nicola
虽然多种基因型的空肠弯曲菌可能是从同一个商业肉鸡群中分离出来的，但人们对不同基因型的感染动力学及其在肠道内的定植部位知之甚少。单次实验感染C. jejuniM 1（序列类型137，克隆复合体45）和C.空肠13126（序列类型21，克隆复合体21）显示13126以显著更高的水平定殖于盲肠。两种C.然后在实验肉鸡群中检查空肠菌株。两只33日龄肉鸡感染M1，两只感染13126，15只鸡未受攻击。感染后取泄殖腔拭子，以确定每种菌株的定殖和脱落。到感染后2天（dpi），8/19只鸡散毒M1，而无鸡散毒13126。在8 dpi时，所有鸡都在脱落两种毒株。在18 dpi时，对每种分离株的肝脏和盲肠水平进行定量，而在10只鸡中，还在整个胃肠道（GI）的9个部位对它们进行定量。在整个胃肠道中发现了13126，而M1主要限于盲肠和结肠。7/ 19只鸡的肝脏仅对13126培养呈阳性。这些数据表明，13126具有与菌株M1明显不同的感染生物学。它表现出较低的胃肠道定植速度较慢，但更具侵袭性，能够在整个胃肠道以高水平定植。C.空肠菌株在鸡体内具有明显不同的感染生态，这对家禽业的控制具有意义，并表明可食用组织的污染风险取决于所涉及的分离株。
Although multiple genotypes of Campylobacter jejuni may be isolated from the same commercial broiler flock, little is known about the infection dynamics of different genotypes within individuals or their colonization sites within the gut. Single experimental infections with C. jejuni M1 (sequence type 137, clonal complex 45) and C. jejuni 13126 (sequence type 21, clonal complex 21) revealed that 13126 colonized the ceca at significantly higher levels. The dissemination and colonization sites of the two C. jejuni strains then were examined in an experimental broiler flock. Two 33-day-old broiler chickens were infected with M1 and two with 13126, and 15 birds were left unchallenged. Cloacal swabs were taken postinfection to determine the colonization and shedding of each strain. By 2 days postinfection (dpi), 8/19 birds were shedding M1 whereas none were shedding 13126. At 8 dpi, all birds were shedding both strains. At 18 dpi, liver and cecal levels of each isolate were quantified, while in 10 birds they also were quantified at nine sites throughout the gastrointestinal (GI) tract. 13126 was found throughout the GI tract, while M1 was largely restricted to the ceca and colon. The livers of 7/ 19 birds were culture positive for 13126 only. These data show that 13126 has a distinctly different infection biology than strain M1. It showed slower colonization of the lower GI tract but was more invasive and able to colonize at a high level throughout the GI tract. The finding that C. jejuni strains have markedly different infection ecologies within the chicken has implications for control in the poultry industry and suggests that the contamination risk of edible tissues is dependent on the isolate involved.