Identification and characterization of a Sox2 homolog in the Japanese flounder Paralichthys olivaceus
Identification and characterization of a Sox2 homolog in the Japanese flounder Paralichthys olivaceus
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日本牙鲆中 Sox2 同源物的鉴定和表征
DOI：
10.1016/j.gene.2014.04.062
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发表时间：
2014-07-10
期刊：
影响因子：
3.5
通讯作者：
Zhang, Quanqi
中科院分区：
文献类型：
--
作者：
Gao, Jinning;Wang, Zhongkai;Zhang, Quanqi
SOX2在维持胚胎干细胞和神经干细胞的多能性以及神经发生中起着重要作用。虽然它在哺乳动物中已经得到了很好的研究，但来自低等无脊椎动物，特别是海鱼的信息仍然有限。在本研究中，我们克隆并测序了比目鱼Sox2的全长cDNA和部分5‘-侧翼区。系统发育、基因结构和蛋白质比较分析表明，牙鲆Sox2(Po-Sox2)与哺乳动物Sox2同源。实时荧光定量RT-PCR结果表明，Po-Sox2不是母系遗传，转录本从高胚胎期开始存在，原肠中期表达水平最高。组织分布分析表明，Po-Sox2不仅存在于神经组织中，也存在于性腺和鳃组织中。此外，我们还分析了Po-Sox2启动子区域的几个物种保守基序以及各种转录因子结合位点。CpG在5‘-调控区的整体低甲基化状态表明它不参与Po-Sox2的转录调控。这些结果表明，Po-Sox2在神经发生和早期胚胎发育中可能具有保守的功能。(C)2014爱思唯尔B.V.保留所有权利。
Sox2 plays an essential role in maintaining the pluripotency of embryonic and neural stem cells as well as in the neurogenesis. While it has been well studied in mammals, information from lower invertebrate especially marine fish is still limited. In this study, we cloned and sequenced the full-length cDNA and partial 5'-flanking region of the Japanese flounder Sox2. Phylogenetic, gene structure, and protein comparison analyses revealed that Paralichthys olivaceus Sox2 (Po-Sox2) was homologous to mammalian Sox2. Quantitative real-time RT-PCR results showed that Po-Sox2 was not maternal inherited, and the transcripts were present from high blastula-stage onwards, with the highest level at the mid-gastrula stage. Tissue distribution analysis revealed that Po-Sox2 was present not only in neural tissues, but also in gonadal and gill tissues. In addition, we analyzed the Po-Sox2 promoter region for several species-conserved motifs as well as various transcription factor binding sites. The overall hypomethylation status of the identified CpG sites in the 5'-regulatory region revealed that it was not involved in the transcriptional modulation of Po-Sox2. All these results suggest that Po-Sox2 may have a conserved function in neurogenesis and early embryonic development. (C) 2014 Elsevier B.V. All rights reserved.