Identification, classification, and transcription profiles of the B-type response regulator family in pear.
Identification, classification, and transcription profiles of the B-type response regulator family in pear.
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豌豆 B 型反应调节家族的鉴定、分类和转录谱
DOI：
10.1371/journal.pone.0171523
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Teng Y
中科院分区：
文献类型：
--
作者：
Ni J;Bai S;Gao L;Qian M;Zhong L;Teng Y
B型反应调节因子（B-RRs）是在双组分信号系统的最后一步发挥作用的转录因子。在模式植物中，B-RRs已被证明在细胞分裂素信号转导中起重要作用。然而，B-RRs在梨中的功能尚未得到很好的研究。在这份报告中，我们进行了全基因组分析，并确定了11个推定的基因编码的B-PpRR蛋白的基础上公布的基因组序列的梨。构建了B-PpRR家族的系统发育树，并确定了B-PpRR家族基因的基序分布、染色体定位和基因结构。从转录组数据确定的基因转录谱表明，B-PpRR基因可能在梨果实发育、芽休眠和光/光诱导的花青素积累过程中发挥作用。用细胞分裂素N-（2-氯-4-吡啶基）- N′-苯基脲（CPPU）处理从不积累花青苷的翠冠梨幼果，可明显诱导花青苷的积累。CPPU处理后16 d，果皮中的花青苷开始积累，沿着大部分花青苷生物合成基因的激活。对B-PpRR转录水平的分析表明，B-PpRR基因介导了花青素的积累。这些结果丰富了我们对B-PpRR基因在梨生理过程中功能的理解。
Type-B response regulators (B-RRs) are transcription factors that function in the final step of two-component signaling systems. In model plants, B-RRs have been shown to play important roles in cytokinin signal transduction. However, the functions of B-RRs in pear have not been well studied. In this report, we conducted a genome-wide analysis and identified 11 putative genes encoding B-PpRR proteins based on the published genome sequence of Pyrus bretschneideri. A phylogenetic tree of the B-PpRR family was constructed, and the motif distribution, chromosome localization, and gene structure of B-PpRR family genes were determined. Gene transcript profiles, which were determined from transcriptome data, indicated that B-PpRR genes potentially function during pear fruit development, bud dormancy, and light/hormone-induced anthocyanin accumulation. Treatment of the fruitlets of ‘Cuiguan’ pear (Pyrus pyrifolia), which never accumulates anthocyanin, with the cytokinin N-(2-chloro-4-pyridyl)- N′-phenylurea (CPPU) clearly induced anthocyanin accumulation. Anthocyanins accumulated in the skin of fruitlets by 16 days after CPPU treatment, along with the significant activation of most anthocyanin biosynthetic genes. Analyses of B-PpRR transcript levels suggested that B-PpRR genes mediated this accumulation of anthocyanins. These findings enrich our understanding of the function of B-PpRR genes in the physiological processes of pear.