Biosynthesis of vitamin B(6) in yeast: incorporation pattern of glucose.
Biosynthesis of vitamin B(6) in yeast: incorporation pattern of glucose.
复制标题
酵母中维生素 B(6) 的生物合成：葡萄糖的掺入模式。
DOI：
10.1021/ja0113201
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发表时间：
2001
影响因子：
15
通讯作者：
Spenser,ID
中科院分区：
文献类型：
--
作者：
Gupta,RN;Hemscheidt,T;Sayer,BG;Spenser,ID
Two yeasts,Saccharomyces cerevisiaeATCC 7752 andCandidautilisATCC 9256, were incubated in the presence of variously multiply13C-labeled samples ofd-glucose. The13C incorporation pattern within pyridoxamine dihydrochloride, established by13C NMR spectroscopy, differed from that which had previously been found within pyridoxine, isolated fromEscherichia coli. Thus, the origin of the carbon skeleton of vitamin B6in yeast differs substantially from its origin inE. coli. In particular, in yeast the distribution of13C within the C5chain C-2‘,2,3,4,4‘ of pyridoxamine corresponds to the distribution of13C within the C5chain C-1,2,3,4,5 of the ribose component of cytidine. It follows that the C5chains of pyridoxamine and of ribose originate from a common glucose-derived pentulose or pentose intermediate. By contrast, inE. colithe C5chain of pyridoxine is derived from 1-deoxy-d-xylulose 5-phosphate which, in turn, originates by condensation of pyruvic acid with glyceraldehyde 3-phosphate.
