Prevention of short-term ultraviolet B radiation-mediated damages by resveratrol in SKH-1 hairless mice
Prevention of short-term ultraviolet B radiation-mediated damages by resveratrol in SKH-1 hairless mice
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DOI：
10.1016/s0041-008x(02)00014-5
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发表时间：
2003-01-01
影响因子：
3.8
通讯作者：
Ahmad, N
中科院分区：
文献类型：
--
作者：
Afaq, F;Adhami, VM;Ahmad, N
非黑色素瘤皮肤癌是人类最常见的癌症，太阳紫外线辐射，特别是紫外线成分(290-320 nm)是其主要原因。减少癌症发生的一种方法是使用被称为“光化学预防药物”的物质(通常是抗氧化剂)。白藜芦醇(反式-3，4‘，5-三羟基二苯乙烯)是一种植物抗毒素，存在于葡萄、坚果、水果和红葡萄酒中，是一种有效的抗氧化剂，具有很强的抗炎和抗增殖特性。这项研究旨在检验白藜芦醇是否具有改善短期UVB暴露于小鼠皮肤所造成的损害的潜力。对SKH-1无毛小鼠单独局部应用白藜芦醇(每只小鼠25摩尔/0.2毫升丙酮)可显著抑制UVB(180mJ/cm(2))介导的皮肤增厚和皮肤水肿。白藜芦醇对小鼠皮肤的作用也被发现显著抑制了UVB诱导的环氧合酶和鸟氨酸脱羧酶(ODC)的活性和蛋白表达，这两种酶是肿瘤促进的成熟标志物。我们还观察到，白藜芦醇抑制UVB介导的脂质过氧化水平的增加，这是氧化应激的标志。综上所述，我们的结果表明，白藜芦醇可能对UVB暴露造成的损害具有实质性的保护作用。而这些保护作用可能是通过其抗氧化特性来实现的。(C)2003年埃尔塞维尔科学公司(美国)。版权所有。
Nonmelanoma skin cancer is the most common cancer among humans and solar UV radiation, particularly it,, UVB component (290-320 nm), is its major cause. One way to reduce the occurrence of the cancer is via the use Of substances (often antioxidants) termed "photochemopreventive agents". Resveratrol (trans-3,4',5-trihydroxystilbene), a phytoalexin found in grapes, nuts, fruits, and red wine, is a potent antioxidant with strong anti-inflammatory and antiproliferative properties. This studs was designed to examine whether resveratrol possesses the potential to ameliorate the damages caused by short-term UVB exposure to mouse skin. Single topical application of resveratrol (25 mumol/0.2 ml acetone per mouse) to SKH-1 hairless mice was found to result in significant inhibition of UVB (180 mJ/cm(2))-mediated increase in bifold skin thickness and skin edema. The resveratrol treatment to mouse skin was also found to result in significant inhibition of UVB-mediated induction of cyclooxygenase and ornithine decarboxylase (ODC) enzyme activities and protein expression of ODC, which are well-established markers for tumor promotion. We also observed that resveratrol inhibits UVB-mediated increased level of lipid peroxidation, a marker of oxidative stress. Taken together, our results suggest that resveratrol may afford substantial protection against the damages caused by UVB exposure. and these protective effects may he mediated via its antioxidant properties. (C) 2003 Elsevier Science (USA). All rights reserved.