Expression of tissue factor in non-small-cell lung cancers and its relationship to metastasis.
Expression of tissue factor in non-small-cell lung cancers and its relationship to metastasis.
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DOI：
10.1038/sj.bjc.6690073
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发表时间：
1999-02
影响因子：
8.8
通讯作者：
Yoshizawa, Y
中科院分区：
文献类型：
--
作者：
Sawada, M;Miyake, S;Ohdama, S;Matsubara, O;Masuda, S;Yakumaru, K;Yoshizawa, Y
组织因子（TF）是外源性凝血级联的引发剂。尽管最近的研究揭示了一些肿瘤细胞的转移特性与 TF 表达之间的关系，但 TF 在肺癌，特别是非小细胞肺癌 (NSCLC) 中的意义仍不清楚。在本研究中，通过功能研究、蛋白质印迹分析和免疫细胞化学染色在 NSCLC 细胞系中检测到 TF。还通过酶联免疫吸附测定 (ELISA) 定量了 8 个 NSCLC 细胞系中的 TF 水平，并评估了 55 个手术切除的 NSCLC 样本中的 TF 表达。源自转移性病变的 NSCLC 细胞系产生高水平的 TF（48.3 ± 23.5 ng 10−6 细胞，平均值 ± s.e.m.），而源自原发性病变的 NSCLC 细胞系产生低水平的 TF（0.2 ± 0.1 ng 10−6 细胞）。免疫组织化学研究表明，转移的 NSCLC 患者细胞中的 TF 染色明显强于未转移的细胞。在 28 例转移患者中，TF 强阳性 10 例，中度阳性 16 例，阴性 2 例。相比之下，在 27 名无转移患者中，只有 2 名 TF 强阳性，18 名中度阳性，7 名 TF 阴性。因此，肺癌患者的恶性细胞会产生不同水平的TF，TF可能在转移过程中发挥重要作用。 © 1999 癌症研究运动
Tissue factor (TF) is an initiator of the extrinsic cascade of blood coagulation. Although recent studies have revealed a relationship between metastatic properties and TF expression in some neoplastic cells, the significance of TF in lung cancer, especially in non-small-cell lung cancer (NSCLC), is still unclear. In this study, TF was detected in NSCLC cell lines by functional study, Western blot analysis and immunocytochemical staining. TF levels in eight NSCLC cell lines were also quantitated by enzyme-linked immunosorbent assay (ELISA), and TF expression was evaluated in 55 specimens of surgically resected NSCLCs. NSCLC cell lines derived from metastatic lesions produced high levels of TF (48.3 ± 23.5 ng 10−6 cells, mean ± s.e.m.), whereas those derived from primary lesions produced low levels of TF (0.2 ± 0.1 ng 10−6 cells). Immunohistochemical studies disclosed significantly stronger staining for TF in cells from NSCLC patients with metastasis than in those without metastasis. Among the 28 patients with metastasis, ten were strongly positive, 16 were moderately positive and two were negative for TF. In contrast, among the 27 patients without metastasis, only two were strongly positive, 18 were moderately positive and seven were negative for TF. Therefore, malignant cells from patients with lung cancer produce various levels of TF, and TF may play an important role in the metastatic process. © 1999 Cancer Research Campaign