Apoptosis-related proteins (Fas, Fas ligand, bcl-2 and p53) in different types of human breast tumors.
Apoptosis-related proteins (Fas, Fas ligand, bcl-2 and p53) in different types of human breast tumors.
复制标题
DOI：
10.3892/or.9.5.977
复制
发表时间：
2002-09
期刊：
影响因子：
4.2
通讯作者：
H. Ben‐Hur;Eitan Mordechay;R. Halperin;P. Gurevich;J. Zandbank;Meherdad Herper;I. Zusman
中科院分区：
文献类型：
--
作者：
H. Ben‐Hur;Eitan Mordechay;R. Halperin;P. Gurevich;J. Zandbank;Meherdad Herper;I. Zusman
本研究旨在探讨凋亡相关蛋白（阿普：Fas和Fas配体、bcl-2、p53）在乳腺癌发生发展中的作用。本文对50例不同类型的乳腺肿瘤进行了上皮肿瘤细胞和淋巴细胞的免疫组化分析，以确定其淋巴样浸润率和阿普的存在。肿瘤包括：i）纤维囊性疾病（n=12）; ii）良性纤维腺瘤（n=11）; iii）原位癌（n=8）; iv）浸润性导管癌伴高淋巴浸润（n=12）;和v）浸润性导管癌伴淋巴耗竭（n=7）。纤维囊性病和纤维腺瘤都有少量的淋巴细胞和非常少的淋巴浸润。在原位癌中，淋巴浸润的扩大和淋巴细胞密度的增加导致淋巴细胞总数的增加，反映了免疫反应的增强。在淋巴细胞高度浸润的癌中，Fas、FasL和p53阳性细胞明显增多，bcl-2阳性细胞无明显变化，而bcl-2阳性淋巴细胞减少。在淋巴细胞耗竭的癌中，由于淋巴系统的深亚代偿，发现了相反的情况。阿普存在于数量显著高于上皮肿瘤细胞的淋巴细胞中。这表明肿瘤中启动细胞凋亡的细胞本身也受到了该过程的损害。乳腺恶性肿瘤中淋巴细胞的强烈凋亡可能是乳腺肿瘤进展的原因之一。
The aim of this study was to evaluate the possible role of apoptosis-related proteins (ARP: Fas and Fas ligand, bcl-2, p53) in the progress of tumorigenesis in breast cancer. Epithelial tumor cells and lymphocytes were analyzed immunohistochemically for the rate of lymphoid infiltration and presence of ARP in 50 human breast tumors of different types. The tumors included: i) fibrocystic disease (n=12); ii) benign fibroadenoma (n=11); iii) carcinoma in situ (n=8); iv) invasive ductal carcinoma with high lymphoid infiltration (n=12); and v) invasive ductal carcinoma with lymphoid depletion (n=7). Both fibrocystic disease and fibroadenomas had low amounts of lymphocytes and very little lymphoid infiltration. In cancer in situ, expansion of lymphoid infiltrates and increased density of lymphocytes resulted in a rise in the total number of lymphocytes, reflecting intensification of the immune response. In carcinomas with high lymphoid infiltration, a significant increase in the number of Fas and FasL and p53-positive cells was found. The number of bcl-2-positive tumor cells in these tumors was not changed, whereas the number of bcl-2-positive lymphocytes decreased. In carcinomas with lymphoid depletion, the opposite picture was found as a result of deep subcompensation of the lymph system. ARP are present in a significantly higher number of lymphocytes than of the epithelial tumor cells. This indicates that the cells initiating apoptosis in tumors are themselves damaged by the process. The intense apoptosis in lymphocytes in malignant tumors may be one of the reasons for the progress of breast tumors.