Du-Huo-Ji-Sheng-Tang Attenuates Inflammation of TNF-Tg Mice Related to Promoting Lymphatic Drainage Function.
Du-Huo-Ji-Sheng-Tang Attenuates Inflammation of TNF-Tg Mice Related to Promoting Lymphatic Drainage Function.
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DOI：
10.1155/2016/7067691
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Liang Q
中科院分区：
文献类型：
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作者：
Chen Y;Li J;Li Q;Wang T;Xing L;Xu H;Wang Y;Shi Q;Zhou Q;Liang Q
为探讨独活寄生汤（DHJST）对RA淋巴引流功能相关炎症反应的影响，我们用DHJST（12 g/kg）或等体积生理盐水处理8只3月龄TNF-Tg小鼠，每天1次，共12周，并以3月龄WT同窝小鼠作为阴性对照。十二周后，我们进行了近红外ICG成像，发现DHJST增加了ICG在足垫的清除率和腘淋巴结与足垫之间的输出淋巴管的搏动。踝关节的组织学染色显示DHJST减少TNF-Tg小鼠的滑膜炎症、骨侵蚀、软骨侵蚀和TRAP+破骨细胞面积。抗Lyve-1和podoplanin抗体免疫组化染色显示DHJST刺激TNF-Tg小鼠踝关节淋巴管生成。斑马鱼实验表明DHJST促进胸淋巴管的形成。结论：DHJST可抑制TNF-Tg小鼠的炎症程度，促进淋巴管生成和淋巴引流功能。
To investigate whether Du-Huo-Ji-Sheng-Tang (DHJST) attenuate inflammation of RA related to lymphatic drainage function in vivo, we treated eight 3-month-old TNF-Tg mice with DHJST (12 g/kg) or the same volume of physiological saline once every day for 12 weeks, and 3-month-old WT littermates were used as negative control. After twelve weeks, we performed NIR-ICG imaging and found that DHJST increased the ICG clearance at the footpad and the pulse of efferent lymphatic vessel between popliteal lymph node and footpad. Histology staining at ankle joints showed that DHJST decreases synovial inflammation, bone erosion, cartilage erosion, and TRAP+ osteoclast area in TNF-Tg mice. Immunohistochemical staining by using anti-Lyve-1 and anti-podoplanin antibody showed that DHJST stimulated lymphangiogenesis in ankle joints of TNF-Tg mice. And zebrafish study suggested that DHJST promoted the formation of lymphatic thoracic duct. In conclusion, DHJST inhibits inflammation severity and promotes lymphangiogenesis and lymphatic drainage function of TNF-Tg mice.