HOMOLOGS OF A VACUOLAR PROCESSING ENZYME THAT ARE EXPRESSED IN DIFFERENT ORGANS IN ARABIDOPSIS-THALIANA
HOMOLOGS OF A VACUOLAR PROCESSING ENZYME THAT ARE EXPRESSED IN DIFFERENT ORGANS IN ARABIDOPSIS-THALIANA
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DOI：
10.1007/bf00019120
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发表时间：
1995-10-01
影响因子：
5.1
通讯作者：
HARANISHIMURA, I
中科院分区：
文献类型：
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作者：
KINOSHITA, T;NISHIMURA, M;HARANISHIMURA, I
液泡处理酶(VPEs)负责种子蛋白质的成熟。这些加工酶属于一类新的半胱氨酸蛋白酶，分子量在37-39 kDa之间。我们从拟南芥基因组文库中分离到两个VPEs基因。基因产物命名为α-VPE和β-VPE，氨基酸序列同源性为56%。α-VPE和β-VPE的氨基酸序列与蓖麻籽VPE的同源性分别为55%和67%。α-VPE基因有7个内含子，而β-VPE基因有8个内含子。Northern印迹分析表明，α-VPE在拟南芥的丛生叶、茎生叶和茎中表达，而β-VPE主要在花和芽中表达。角果中不表达α-VPE和β-VPE。这一结果强烈表明，分离的基因编码VPE同工酶，这种同工酶是营养器官所特有的。
Vacuolar processing enzymes (VPEs) are responsible for the maturation of seed proteins. These processing enzymes belong to a novel group of cysteine proteinases with molecular masses of 37 to 39 kDa. We isolated two genes of VPEs from a genomic library of Arabidopsis. The gene products were designated alpha-VPE and beta-VPE, and they were 56% identical in terms of amino acid sequence. The amino acid sequences of alpha-VPE and beta-VPE were also 55% and 67% identical to that of castor bean VPE, respectively. The gene for alpha-VPE had 7 introns, while that of beta-VPE had 8 introns. Northern blot analysis revealed that alpha-VPE is expressed in rosette leaves, cauline leaves and stems of Arabidopsis, while beta-VPE is predominantly expressed in the flowers and buds. Neither alpha-VPE nor beta-VPE is expressed in the siliques. This result strongly suggests that the isolated genes encode isozymes of VPE that are specific to vegetative organs.