Controllable labelling of stem cells with a novel superparamagnetic iron oxide-loaded cationic nanovesicle for MR imaging
Controllable labelling of stem cells with a novel superparamagnetic iron oxide-loaded cationic nanovesicle for MR imaging
复制标题
用新型超顺磁性氧化铁负载阳离子纳米囊泡对干细胞进行可控标记，用于磁共振成像
DOI：
10.1007/s00330-012-2509-z
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发表时间：
2012-11-01
影响因子：
5.9
通讯作者：
Shuai, Xin Tao
中科院分区：
文献类型：
--
作者：
Guo, Ruo Mi;Cao, Nuo;Shuai, Xin Tao
探讨高效、可控干细胞标记细胞MRI的可行性。合成了一种新型阳离子超顺磁性氧化铁纳米颗粒（SPION），用于标记大鼠骨髓间充质干细胞，无需二次转染剂。评估最佳标记条件和可控性，并确定标记对细胞活力、增殖活性和多系分化的影响。18只大鼠诱导局灶性缺血性脑损伤，随机注射1 × 10(6)个标记为0、8或20 mv纳米囊泡的细胞（每个n = 6）。对侧纹状体移植细胞进行体内MRI观察，结果与组织学相关。细胞标记的最佳条件为浓度为3.15 μ g Fe/mL的纳米囊泡，带20 mv正电荷，孵育时间为1 h。标记效率随纳米囊泡电势的增加呈线性变化。标记不影响细胞活力、增殖活性或多系分化能力。MRI可检测标记细胞的分布和迁移。组织学证实移植的细胞保留了标记并保持活力。干细胞可以有效和安全地用阳离子、装载spion的纳米囊泡以可控的方式进行细胞MRI标记。干细胞可以用阳离子、装载spion的纳米囊泡进行有效标记。标记不影响细胞活力、增殖或分化。利用阳离子纳米囊泡可以控制SPION的细胞摄取。标记细胞可沿胼胝体向脑梗死方向迁移。移植的、标记过的细胞保留了标记并保持活力。
To investigate the feasibility of highly efficient and controllable stem cell labelling for cellular MRI.A new class of cationic, superparamagnetic iron oxide nanoparticle (SPION)-loaded nanovesicles was synthesised to label rat bone marrow mesenchymal stem cells without secondary transfection agents. The optimal labelling conditions and controllability were assessed, and the effect of labelling on cell viability, proliferation activity and multilineage differentiation was determined. In 18 rats, focal ischaemic cerebral injury was induced and the rats randomly injected with 1 x 10(6) cells labelled with 0-, 8- or 20-mV nanovesicles (n = 6 each). In vivo MRI was performed to follow grafted cells in contralateral striata, and results were correlated with histology.Optimal cell labelling conditions involved a concentration of 3.15 mu g Fe/mL nanovesicles with 20-mV positive charge and 1-h incubation time. Labelling efficiency showed linear change with an increase in the electric potentials of nanovesicles. Labelling did not affect cell viability, proliferation activity or multilineage differentiation capacity. The distribution and migration of labelled cells could be detected by MRI. Histology confirmed that grafted cells retained the label and remained viable.Stem cells can be effectively and safely labelled with cationic, SPION-loaded nanovesicles in a controllable way for cellular MRI.aEuro cent Stem cells can be effectively labelled with cationic, SPION-loaded nanovesicles.aEuro cent Labelling did not affect cell viability, proliferation or differentiation.aEuro cent Cellular uptake of SPION could be controlled using cationic nanovesicles.aEuro cent Labelled cells could migrate along the corpus callosum towards cerebral infarction.aEuro cent The grafted, labelled cells retained the label and remained viable.