Analysis of simple sequence repeat markers linked with blast disease resistance genes in a segregating population of rice (Oryza sativa)
Analysis of simple sequence repeat markers linked with blast disease resistance genes in a segregating population of rice (Oryza sativa)
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DOI：
10.4238/vol10-3gmr1331
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发表时间：
2011-01-01
影响因子：
0.4
通讯作者：
Latif, M. A.
中科院分区：
文献类型：
--
作者：
Ashkani, S.;Rafii, M. Y.;Latif, M. A.
在120个SSR标记中，利用23个多态性标记对来自抗稻品种Pongsu Seribu 2号和感稻品种Mahsuri的F2群体的320个个体进行了分离率的鉴定。在表型研究方面，利用稻瘟病菌致病力最强的致病型P7.2对F-2群体进行筛选，以了解稻瘟病抗性的遗传规律，并利用SSR标记进行连锁分析。只有11个标记与单基因模型（d.f. = 1.0，P < 0.05）。在表型数据分析中，F-2群体中的抗病和感病植株分别以3：1（R：S）的比例分离。因此，Pongsu Seribu 2对稻瘟病致病型P7.2的抗性很可能是由一个核基因控制的。F2代抗P7.2的植株与RM 168（116 bp）、RM 8225（221 bp）、RM 1233（175 bp）、RM 6836（240 bp）、RM 5961（129 bp）和RM 413（79 bp）等6个SSR标记连锁。这些诊断标记可用于标记辅助选择程序，以开发持久的抗稻瘟病品种。
Among 120 simple sequence repeat (SSR) markers, 23 polymorphic markers were used to identify the segregation ratio in 320 individuals of an F-2 rice population derived from Pongsu Seribu 2, a resistant variety, and Mahsuri, a susceptible rice cultivar. For phenotypic study, the most virulent blast (Magnaporthe oryzae) pathotype, P7.2, was used in screening of F-2 population in order to understand the inheritance of blast resistance as well as linkage with SSR markers. Only 11 markers showed a good fit to the expected segregation ratio (1:2:1) for the single gene model (d.f. = 1.0, P < 0.05) in chi-square (chi(2)) analyses. In the phenotypic data analysis, the F-2 population segregated in a 3:1 (R:S) ratio for resistant and susceptible plants, respectively. Therefore, resistance to blast pathotype P7.2 in Pongsu Seribu 2 is most likely controlled by a single nuclear gene. The plants from F-2 lines that showed resistance to blast pathotype P7.2 were linked to six alleles of SSR markers, RM168 (116 bp), RM8225 (221 bp), RM1233 (175 bp), RM6836 (240 bp), RM5961 (129 bp), and RM413 (79 bp). These diagnostic markers could be used in marker assisted selection programs to develop a durable blast resistant variety.