Fates-shifted is an F-box protein that targets Bicoid for degradation and regulates developmental fate determination in Drosophila embryos.
Fates-shifted is an F-box protein that targets Bicoid for degradation and regulates developmental fate determination in Drosophila embryos.
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DOI：
10.1038/ncb2141
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发表时间：
2011-01
影响因子：
21.3
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Bicoid （Bcd）是一种形态发生蛋白，在果蝇胚胎中指导前后轴（a -p）方向的模式。尽管进行了广泛的研究，但是什么控制了Bcd正常浓度梯度的形成仍然是一个未解决和有争议的问题。在本报告中，我们发现Bcd蛋白的降解是通过泛素-蛋白酶体途径介导的。我们发现了一种新的F-box蛋白，由命运转移（fsd）编码，它通过靶向Bcd蛋白泛素化在Bcd蛋白降解中起重要作用。缺少fsd的雌性胚胎会改变Bcd梯度，导致命运图沿a - p轴移动。我们的研究代表了第一个实验证明，与另一个假设相反，Bcd蛋白降解是正常梯度形成和发育命运决定所必需的。
Bicoid (Bcd) is a morphogenetic protein that instructs patterning along the anterior-posterior (A-P) axis in Drosophila embryos. Despite extensive studies, what controls the formation of a normal concentration gradient of Bcd remains an unresolved and controversial question. In this report we show that Bcd protein degradation is mediated by the ubiquitin-proteasome pathway. We identify a novel F-box protein, encoded by fates-shifted (fsd), that plays an important role in Bcd protein degradation by targeting it for ubiquitination. Embryos from females lacking fsd have an altered Bcd gradient profile, resulting in a shift of the fatemap along the A-P axis. Our study represents a first experimental demonstration that, contrary to an alternative hypothesis, Bcd protein degradation is required for normal gradient formation and developmental fate determination.