Toward a description of the sialome of the adult female mosquito Aedes aegypti
Toward a description of the sialome of the adult female mosquito Aedes aegypti
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DOI：
10.1016/s0965-1748(02)00047-4
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发表时间：
2002-09-01
影响因子：
3.8
通讯作者：
Ribeiro, JMC
中科院分区：
文献类型：
--
作者：
Valenzuela, JG;Pham, VM;Ribeiro, JMC
为了描述埃及伊蚊唾液腺 (sialoome) 中表达的 mRNA 和蛋白质组，我们随机测序了该昆虫的全长 cDNA 文库，并对唾液匀浆中的 PVDF 转移蛋白条带进行了 Edman 降解。我们发现了 23 8 个 cDNA 簇，其中包含编码通过氨基末端降解发现的 11 种蛋白质中的 10 种的簇。之前描述的所有六种唾液蛋白均在该文库中发现。报道了 32 个新 cDNA 序列的全长序列，其中之一是转座元件的产物。在 31 个新蛋白质序列中，有 4 个 D7 蛋白质家族成员；抗原5家族的4个新成员（伊蚊中未报道的蛋白质家族）；新颖的丝氨酸蛋白酶抑制剂； 30-kDa 的 Ae 过敏原的新成员。埃及伊蚊；分泌性钙网蛋白； 2种与哺乳动物血管生成素相似的蛋白质；腺苷脱氨酶；嘌呤水解酶；溶菌酶； C型凝集素； 3种丝氨酸蛋白酶，其中一种与家蚕酚氧化酶激活酶高度相似； 2 与无脊椎动物免疫相关的蛋白质；以及一些与已知蛋白质没有显着匹配的序列。讨论了这些蛋白质在蚊子喂养血液和糖中的可能作用。由爱思唯尔科学有限公司出版
To describe the set of mRNA and protein expressed in the salivary glands (sialome) of Aedes aegypti mosquitoes, we randomly sequenced a full-length cDNA library of this insect and performed Edman degradation of PVDF-transferred protein bands from salivary homogenates. We found 23 8 cDNA clusters which contained those coding for 10 of the 11 proteins found by aminoterminal degradation. All six previously described salivary proteins were found in this library. Full-length sequences of 32 novel cDNA sequences are reported, one of which is the product of a transposable element. Among the 31 novel protein sequences are 4 additional members of the D7 protein family; 4 novel members of the antigen 5 family (a protein family not reported in Aedes); a novel serpin; a novel member of the 30-kDa allergen of Ae. Aegypti; a secreted calreticulin; 2 proteins similar to mammalian angiopoietins; adenosine deaminase; purine hydrolase; lysozyme; a C-type lectin; 3 serine proteases, including one with high similarity to Bombyx prophenoloxidase, activating enzyme; 2 proteins related to invertebrate immunity; and several sequences that have no significant matches to known proteins. The possible role of these proteins in blood and sugar feeding by the mosquito is discussed. Published by Elsevier Science Ltd.