Metal ion dependence of phosphorothioate ATP analogues in the Bacillus stearothermophilus tyrosyl-tRNA synthetase reaction.
Metal ion dependence of phosphorothioate ATP analogues in the Bacillus stearothermophilus tyrosyl-tRNA synthetase reaction.
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嗜热脂肪芽孢杆菌酪氨酰-tRNA 合成酶反应中硫代磷酸 ATP 类似物的金属离子依赖性。
DOI：
10.1021/bi00458a041
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发表时间：
1990
期刊：
影响因子：
2.9
通讯作者：
Fersht,AR
中科院分区：
文献类型：
--
作者：
Garcia,GA;Leatherbarrow,RJ;Eckstein,F;Fersht,AR
Materials and MethodsThe diastereomers of ATPaS and ATP0S were prepared as described previously (Eckstein & Goody, 1976; Yee et al., 1979). The ATP analogues were characterized by 31P NMR spectroscopy and reverse-phase HPLC as previously described for the diastereomers of ATPaS (Ludwig & Eckstein, 1989). For the HPLC analysis of the diastereomers of ATP| 8S, the same system was used except that the triethylammonium bicarbonate buffer contained 20 mM MgCl2 and the aceto-nitrile concentration was linearly increased from 0 to 4.5% over 15 min (Marquetant & Goody, 1983). This led to a partial resolution of the two diastereomers with retentiontimes for the SP isomer of 4.83 min and for the RP isomer of 5.18 min. The 31P NMR spectra of the diastereomers of ATP/3S were identical with those reportedearlier (Sheu & Frey, 1977). The tyrosyl-tRNA synthetase from B. stearothermophilus was expressed in Escherichia coli TG2 hosts from M13mp9 phage constructed as described previously (Carter et al., 1984) and purified to electrophoretic homogeneity according to the method of Wells and Fersht (1986). Uniformly labeled
DOI：
10.1016/0304-4165(80)90062-8
发表时间：
1980-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
AUTRIC, F;FERRAZ, C;DEMAILLE, JG
通讯作者：
DEMAILLE, JG
公羊精子鞭毛质膜中的 [Ca2 Mg2 ]-ATP 酶和活性 Ca2 转运
DOI：
--
发表时间：
1980
期刊：
影响因子：
--
作者：
M. Bradley;I. Forrester
通讯作者：
I. Forrester