HYDROGEN-ION BUFFERS FOR BIOLOGICAL-RESEARCH
HYDROGEN-ION BUFFERS FOR BIOLOGICAL-RESEARCH
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DOI：
10.1016/0003-2697(80)90079-2
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发表时间：
1980-01-01
影响因子：
2.9
通讯作者：
GOOD, NE
中科院分区：
文献类型：
--
作者：
FERGUSON, WJ;BRAUNSCHWEIGER, KI;GOOD, NE
首次报道了5种新的两性氢离子缓冲剂：3-（N-吗啉代）-2-羟基丙磺酸、3-[N-双（羟乙基）氨基]-2-羟基丙磺酸、3-[N-三（羟甲基）甲氨基]-2-羟基丙磺酸、N-羟乙基哌嗪-N“-2-羟基丙磺酸和哌嗪-N，N”-双（2-羟基丙磺酸）二水合物。在哺乳动物组织培养物[人胚肺成纤维细胞WI-38细胞、成人皮肤成纤维细胞、人宫颈癌HeLa细胞和中国仓鼠卵巢细胞]中对这些缓冲液（所有N-取代的3-氨基-2-羟基丙磺酸，pKa在6.9-7.9之间）进行许多严格试验，植物病理学[蛋白核小球藻生长效应和菠菜叶绿体效应]和病毒学[大豆原生质体的豇豆花叶病毒感染]以检测抑制效应。它们似乎相当于或优于迄今为止可用的任何其他缓冲剂。
Five new zwitterionic hydrogen ion buffers are described for the 1st time: 3-(N-morpholino)-2-hydroxypropanesulfonic acid, 3-[N-bis(hydroxyethyl)amino]-2-hydroxypropanesulfonic acid, 3-[N-tris(hydroxymethyl)methylamino]-2-hydroxypropanesulfonic acid, N-hydroxyethylpiperazine-N''-2-hydroxypropanesulfonic acid and piperazine-N,N''-bis(2-hydroxypropanesulfonic acid)dihydrate. These buffers, all N-substituted 3-amino-2-hydroxypropanesulfonic acids, with pKa between 6.9-7.9 were subjected to a number of stringent tests in mammalian tissue culture [human embryonic lung fibroblast WI-38 cells, adult human skin fibroblast cells, human cervical carcinoma HeLa cells and Chinese hamster ovary cells], plant pathology [Chlorella pyrenoidosa growth effects and spinach chloroplast effects] and virology [cowpea mosaic virus infection of soybean protoplasts] to detect inhibitory effects. They appear to be equivalent to or better than any other buffers heretofore available.