Local versus systemic control of numbers of endometrial T cells during pregnancy in sheep
Local versus systemic control of numbers of endometrial T cells during pregnancy in sheep
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DOI：
10.1046/j.1365-2567.2001.01182.x
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发表时间：
2001-03-01
期刊：
影响因子：
6.4
通讯作者：
Hansen, PJ
中科院分区：
文献类型：
--
作者：
Majewski, AC;Tekin, S;Hansen, PJ
绵羊怀孕与子宫内膜中特定T淋巴细胞数量的变化有关。这些变化可能有助于逃避母体免疫排斥的概念，并提示T细胞在胎盘生长、分娩和产后子宫防御感染中可能发挥作用。本实验的目的是评估系统信号(即存在于整个子宫或来自循环的信号，包括分泌到母体血液中的妊娠激素)与局部作用的妊娠信号在调节妊娠期间子宫内膜淋巴细胞数量变化方面的相对重要性。所采取的方法是通过手术将怀孕限制在一个子宫角，并比较两个子宫角之间以及怀孕母羊和去卵巢母羊的两个子宫角之间的淋巴细胞数量差异。与去卵巢绵羊相比，腺上皮内CD45R(+)淋巴细胞数量减少，腔上皮内γ-Delta T细胞数量增加。这些变化发生在单侧怀孕母羊的怀孕和未怀孕的子宫角中。此外，单侧妊娠母羊的两个子宫角之间的淋巴细胞数也没有显著差异。CD25在两个子宫角组织中均无表达。总之，妊娠期间子宫内膜淋巴细胞数量的变化，而不是由于胚胎起源的局部作用信号，是母体或胚胎起源的激素信号的结果，这些激素信号要么直接作用于子宫内膜淋巴细胞，要么刺激子宫内膜诱导影响淋巴细胞动力学的调节分子的合成。
Pregnancy in sheep is associated with changes in numbers of specific T-lymphocyte populations in the uterine endometrium. These changes probably contribute to evasion by the conceptus of maternal immunological rejection and indicate a possible role for T cells in placental growth, parturition and post-parturient uterine defence against infection. The purpose of the present experiment was to evaluate the relative importance of systemic signals (i.e. those present throughout the uterus or from the circulation, including conceptus hormones secreted into the maternal blood) versus locally acting conceptus signals for regulating changes in numbers of endometrial lymphocytes during pregnancy. The approach taken was to surgically confine pregnancy to one uterine horn and compare differences in lymphocyte numbers between the two uterine horns as well as between both horns of pregnant ewes with those of ovariectomized ewes. As compared with ovariectomized ewes, there was a decline in numbers of CD45R(+) lymphocytes within glandular epithelium and an increase in gamma delta T-cell number within the luminal epithelium. These changes occurred in both the pregnant and non-pregnant uterine horns of unilaterally pregnant ewes. Moreover, there were no significant differences in lymphocyte: numbers between the two uterine horns of unilaterally pregnant ewes. Expression of CD25 was absent in tissues from both uterine horns. In conclusion, changes in numbers of endometrial lymphocytes during pregnancy, rather than due to locally acting signals of conceptus origin, are the result of hormonal signals of maternal or conceptus origin that either act directly on endometrial lymphocytes or stimulate the uterine endometrium to induce synthesis of regulatory molecules that affect lymphocyte dynamics.