Multiplexed and fully automated detection of metabolic biomarkers using microdialysis probe.
Multiplexed and fully automated detection of metabolic biomarkers using microdialysis probe.
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DOI：
10.1016/j.snb.2016.07.097
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发表时间：
2017-01
期刊：
影响因子：
--
通讯作者：
Ledden B
中科院分区：
文献类型：
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作者：
Das C;Wang G;Sun Q;Ledden B
我们在这里报告，设计和开发一个自动化的近实时连续检测系统的乳酸，谷氨酸，丙酮酸和葡萄糖使用微透析探针。该系统可自动推动灌注液通过微透析探针（20、100和1000 kDa截留分子量探针），流速为0.5-2 μL/min，液体回收率几乎为100%。从探头收集的微透析液自动分析这四种代谢物生物标志物。它以连续模式运行，每20分钟测量一次所有四种生物标志物。这些不同标志物的动态范围涵盖了创伤性脑损伤的整个临床范围。原型显示所有生物标志物在整个临床范围内的~ 7-10%的低变化，具有~ 95%的相当好的准确性。仪器可连续运行24 h，无需用户干预。对于往返于微透析探针和相关死体积的1 m长管道，探针位点的实际事件与报告浓度的总滞后时间约为1 h。
We report here, the design and development of an automated near real-time continuous detection system for lactate, glutamate, pyruvate and glucose using microdialysis probe. The system developed can automatically push perfusate through microdialysis probe (20, 100 and 1000 kDa MWCO cutoff probe) at low to medium flow rate of 0.5–2 μL/min with almost 100% fluid recovery. The microdialysate collected from the probe is analyzed automatically for these four metabolite biomarkers. It operates in a continuous mode with measurements of all four biomarkers once every 20 min. The dynamic range for these different markers covers the entire clinical range of traumatic brain injury. The prototype shows a low variation of ~ 7–10% across the entire clinical range for all the biomarkers with fairly good accuracy of ~95%. The instrument canrun continuously for 24 h without user intervention. With a long tubing of 1 m to and from the microdialysis probe and associated dead volume, the total lag time for actual event at the probe site versusreported concentration is roughly 1 h.