AgCa-PLGA submicron particles inhibit the growth and colonization of E. Faecalis and P. Gingivalis on dentin through infiltration into dentinal tubules
AgCa-PLGA submicron particles inhibit the growth and colonization of E. Faecalis and P. Gingivalis on dentin through infiltration into dentinal tubules
复制标题
AgCa-PLGA 亚微米颗粒通过浸润牙本质小管抑制牙本质上粪肠球菌和牙龈卟啉单胞菌的生长和定植
DOI：
10.1016/j.ijpharm.2018.09.066
复制
发表时间：
2018-12-01
影响因子：
5.8
通讯作者：
Fan, Bing
中科院分区：
文献类型：
--
作者：
Fan, Wei;Liu, Danfeng;Fan, Bing
如何有效地清除根管和牙周组织的感染，修复感染的牙周骨组织，一直是一个巨大的挑战。聚（丙交酯-共-乙交酯）（PLGA）颗粒由于其生物可降解性、持续药物释放和无毒特性而被用于药物递送。由于银离子（Ag+）具有很强的抗菌能力，而钙离子（Ca 2+）是硬组织再生所必需的，因此将Ag+和Ca 2+包封到PLGA颗粒中以形成AgCa-PLGA亚微米颗粒。本文报道了其理化性质、离子释放、细胞毒性、牙本质小管浸润能力、对粪肠球菌（Enterococcus faecalis，E.粪菌）和牙龈卟啉单胞菌（P. gingivalis）在牙本质上的粘附和生物膜形式以及体外矿化能力。结果表明，AgCa-PLGA粒子包裹了Ag+和Ca 2+，在30 d内可释放出Ag+和Ca 2+，对大肠杆菌有较强的抗菌作用。faecalis和牙龈卟啉单胞菌。AgCa-PLGA微粒经超声激活后可渗入牙本质小管，抑制E.牙本质上的粪便菌和牙龈卟啉单胞菌。AgCa-PLGA颗粒显示无细胞毒性，并诱导羟基磷灰石（HA）样晶体形成。AgCa-PLGA亚微米粒子可能成为牙科和其他相关医疗领域中感染控制和硬组织再生的生物材料。
Eliminating the infection from root canals and periodontal tissues of human teeth as well as the repair of infected periodontal bone has been a great challenge. Poly(lactide-co-glycolide) (PLGA) particles have been used for drug delivery due to their biodegradability, sustained drug release and nontoxic properties. As silver ions (Ag+) exhibit strong antibacterial ability and calcium ions (Ca2+) are essential for hard tissue regeneration, the Ag+ and Ca2+ were encapsulated into the PLGA particles to form AgCa-PLGA submicron particles. The physicochemical properties, ion release, cytotoxicity, ability to infiltrate dentinal tubules, antibacterial effects against Enterococcus faecalis (E. faecalis) and Porphyromonas gingivalis (P. gingivalis) in either planktonic or biofilm forms on dentin as well as in vitro mineralization ability were investigated. Results showed that Ag+ and Ca2+ were encapsulated into the AgCa-PLGA particles, which could release both Ag+ and Ca2+ over 30 days and exhibited strong antibacterial effects against E. faecalis and P. gingivalis. AgCa-PLGA particles could infiltrate into dentinal tubules by ultrasonic activation and inhibit the colonization of E. faecalis and P. gingivalis on dentin. AgCa-PLGA particles showed no cytotoxicity and induced hydroxyapatite (HA)-like crystal formation. AgCa-PLGA submicron particles could become a biomaterial for both infection control and hard tissue regeneration in dentistry and other related medical areas.