miR-143: A Novel Regulator of MyoD Expression in Fast and Slow Muscles of Siniperca chuatsi
miR-143: A Novel Regulator of MyoD Expression in Fast and Slow Muscles of Siniperca chuatsi
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miR-143：鳜快肌和慢肌中 MyoD 表达的新型调节因子
DOI：
10.2174/1566524014666140228100250
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发表时间：
2014-03-01
影响因子：
2.5
通讯作者：
Zhang, J-S
中科院分区：
文献类型：
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作者：
Chen, L.;Wu, P.;Zhang, J-S
鱼的快和慢骨骼肌纤维的分离程度比哺乳动物要大得多。MyoD是维持肌肉中正常纤维类型平衡所必需的。到目前为止，MyoD在控制肌肉表型方面的上游调控因素还知之甚少。在本研究中，我们以鲟鱼为模型系统，证明miR-143的表达与肌肉中MyoD的表达呈负相关。荧光素酶报告实验进一步证实了miR-143与MyoD之间的直接相互作用。抑制miR-143还导致MyoD和FAST肌球蛋白重链基因在体内的表达显著增加。综上所述，我们的研究表明，miRNA参与控制脊椎动物不同类型肌肉纤维的性能。
The fast and slow skeletal muscle fibers in fish are separated to a much greater degree than in mammals. MyoD is required for the maintenance of normal fiber type balance in muscles. So far, the upstream regulatory factors of MyoD in terms of controlling muscle phenotype are poorly understood. In the present study, we used Siniperca chuatsi as a model system and demonstrated that miR-143 expression was negatively correlated with MyoD expression in the fast and slow muscles of S. chuatsi. The luciferase reporter assay further verified the direct interaction between the miR-143 and MyoD. The miR-143 suppression also led to the significant increase in MyoD and fast myosin heavy chain gene expression in vivo. Taken together, our studies indicated that miRNA participates in controlling the performance of different muscle fiber types in vertebrates.