Use of mucin antibodies and cDNA probes to quantify hypersecretion in vivo in human airways.
Use of mucin antibodies and cDNA probes to quantify hypersecretion in vivo in human airways.
复制标题
使用粘蛋白抗体和 cDNA 探针来量化人呼吸道体内的过度分泌。
DOI：
10.1165/ajrcmb.10.5.8179917
复制
发表时间：
1994
影响因子：
6.4
通讯作者：
Basbaum,C
中科院分区：
文献类型：
--
作者：
Steiger,D;Fahy,J;Boushey,H;Finkbeiner,WE;Basbaum,C
Mucus hypersecretion is a prominent feature of the airway's response to injury. The ability to quantitatively detect mucin and mucin mRNA in vivo in human airways would facilitate the determination of safe exposure levels to various air pollutants and the identification of drugs capable of attenuating mucus hypersecretion. To this end, we have developed two assays: an enzyme-linked immunosorbent assay (ELISA) quantifying mucin-like molecules and a polymerase chain reaction (PCR)-based assay quantifying mucin mRNA. These tests are performed on bronchial lavage fluid and epithelial cells brushed from the surfaces of human airways at bronchoscopy. The PCR data are normalized to eliminate potentially confounding effects of nonepithelial cells in the samples. In a study of six smokers and six nonsmokers, the ELISA detected significantly more mucin-like material in the airways of the smokers than of the nonsmokers. The median mucin concentration for the smokers was 52.2 micrograms/ml (range, 16.3 to 4,860.0), whereas that for the nonsmokers was 12.7 micrograms/ml (range, 4.5 to 22.9). The difference between smokers and nonsmokers was statistically significant (P < or = 0.01). The PCR-based test showed a trend for RNA samples from smokers to be enriched (vis-à-vis nonsmokers) in mucin mRNA.
