Streptosporangiumsubfuscum sp. nov., isolated from the rhizosphere of marigold (Tagetes erecta L.)
Streptosporangiumsubfuscum sp. nov., isolated from the rhizosphere of marigold (Tagetes erecta L.)
复制标题
浅色链孢子囊 sp.
DOI：
10.1007/s10482-014-0293-2
复制
发表时间：
2014
期刊：
影响因子：
--
通讯作者：
W. Xiang
中科院分区：
文献类型：
--
作者：
Ying Zhou;Chongxi Liu;Yuejing Zhang;Chuang Li;J. Xing;Lian;Shuyu Zhou;Xiangjing Wang;W. Xiang
从万寿菊根际分离到一株革兰氏阳性丝状细菌，命名为NEAU-TWSJ13T。在黑龙江省，中国东北部采集的植物，并用多相方法表征。该菌株可形成丰富的气生菌丝，分化为球形孢子囊。16S rRNA基因序列相似性分析表明，菌株NEAU-TWSJ13T属于链孢子菌属，与脆性链孢子菌DSM 43847T亲缘关系最近(98.6%)。16S rRNA基因序列的系统发育分析表明，该基因与脆性链霉菌DSM 43847T、接合链孢霉NBRC 110047T(16S rRNA基因相似性为98.4%)和紫色孢子菌DSM 43849T(16S rRNA基因相似性为97.6%)形成了一个系统发育树。结合DNA-DNA杂交结果和一些表型特征表明，NEAU-TWSJ13T菌株可以与脆性链球菌DSM 43847T和接合链球菌NBRC 110047T区分开来。此外，在形态和生理特征上，NEAU-TWSJ13T还可以与紫色链球菌DSM 43849T和其他16S rRNA基因序列具有很高相似性(>；98.0%)的链孢子菌区分开来。因此，新菌株NEAU-TWSJ13T代表了链霉属的一个新种，命名为亚fuscum sp.11月是提议的。模式株为NEAU-TWSJ13T(=CGMCC 4.7146T=DSM=46724T)。
A Gram-stain positive, filamentous bacterial strain, designated strain NEAU-TWSJ13T, was isolated from the rhizosphere of a marigold (Tagetes erecta L.) plant collected in Heilongjiang Province, northeast China, and characterized using a polyphasic approach. The strain was observed to form abundant aerial hyphae differentiated into spherical sporangia. 16S rRNA gene sequence similarity studies showed that strain NEAU-TWSJ13T belongs to the genus Streptosporangium, being most closely related to Streptosporangium fragile DSM 43847T (98.6 %). Phylogenetic analysis of the 16S rRNA gene sequence indicated that it formed a phyletic line with S. fragile DSM 43847T, Streptosporangium jomthongense NBRC 110047T (98.4 % 16S rRNA gene similarity) and Streptosporangium violaceochromogenes DSM 43849T (97.6 % 16S rRNA gene similarity). A combination of DNA–DNA hybridization results and some phenotypic characteristics indicated that strain NEAU-TWSJ13T can be distinguished from S. fragile DSM 43847T and S. jomthongense NBRC 110047T. Moreover, strain NEAU-TWSJ13T can also be differentiated from S. violaceochromogenes DSM 43849T and other Streptosporangium species showing high 16S rRNA gene sequence similarity (>98.0 %) by morphological and physiological characteristics. Therefore, it is proposed that strain NEAU-TWSJ13T represents a novel species of the genus Streptosporangium, for which the name Streptosporangium subfuscum sp. nov. is proposed. The type strain is NEAU-TWSJ13T ( = CGMCC 4.7146T = DSM = 46724T).
DOI：
10.1099/ijs.0.038075-0
发表时间：
2012-03-01
影响因子：
2.8
作者：
Kim, Ok-Sun;Cho, Yong-Joon;Chun, Jongsik
通讯作者：
Chun, Jongsik