In vivo cleavage of a target RNA by copper kanamycin A.: Direct observation by a fluorescence assay

In vivo cleavage of a target RNA by copper kanamycin A.: Direct observation by a fluorescence assay
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DOI:
10.1039/b108439a
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发表时间:
2002-01-01
影响因子:
4.9
通讯作者:
Cowan, JA
Cowan, JA
中科院分区:
化学2区
文献类型:
--
作者:
Chen, CA;Cowan, JA

文献摘要

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建立了一种新的监测RNA靶序列的体内切割化学的荧光测定法,并用于证明氨基糖苷铜介导的细菌细胞中RNA降解的活性。
A novel fluorescence assay to monitor in vivo cleavage chemistry of RNA target sequences has been established and used to demonstrate the activity of copper aminoglycoside mediated degradation of RNA in bacterial cells.