Mixed lymphocyte reaction induced by multiple alloantigens and the role for IL-10 in proliferation inhibition.
Mixed lymphocyte reaction induced by multiple alloantigens and the role for IL-10 in proliferation inhibition.
复制标题
多种同种抗原诱导的混合淋巴细胞反应以及 IL-10 在增殖抑制中的作用。
DOI：
10.4103/2321-3868.126088
复制
发表时间：
2014
期刊：
影响因子：
5.3
通讯作者：
Wu J
中科院分区：
文献类型：
--
作者：
Zhou J;He W;Luo G;Wu J
能够对同种异体抗原作出反应的T细胞的频率异常高。目前尚不清楚T细胞在同一培养物中受到多种主要组织相容性复合体（MHC）不同同种异体抗原刺激时如何反应。在这份报告中，我们研究了潜在的相互作用的T细胞克隆，同时刺激两套完整的MHC不同的同种异体抗原使用混合淋巴细胞反应（MLR）。在该试验中，我们观察到，与用BALB/c或C3 H同种异体细胞刺激的B6细胞相比，用BALB/c（H-2d）和C3 H（H-2k）同种异体细胞刺激的B6淋巴细胞（H-2b）的增殖没有增加，而是减少。两种MHC抗原刺激后，IL-10蛋白和mRNA表达均显著增加，而IL-2、TNF-α、TGF-β1表达无明显差异。此外，Foxp 3 mRNA表达在所有组中相当。总之，我们观察到的抑制效应，在T细胞增殖反应的多个MHC不匹配的同种异体抗原在MLR，这种效果可能与IL-10的表达上调。
The frequency of T cells that can respond to alloantigens is unusually high. It remains unclear how T cells would respond when stimulated by multiple major histocompatibility complex (MHC) disparate alloantigens in the same cultures. In this report, we examined potential interactions of T cell clones that were stimulated simultaneously by two sets of complete MHC disparate alloantigens using mixed lymphocyte reaction (MLR). In this assay, we observed that proliferation of B6 lymphocytes (H-2b) stimulated by both BALB/c (H-2d) and C3H (H-2k) allogeneic cells was not increased but rather reduced as compared to B6 cells stimulated with either BALB/c or C3H allogeneic cells. Interestingly, interleukin (IL)-10 expressions at both protein level and mRNA level was significantly increased in cultures stimulated with the two MHC alloantigens, while IL-2, tumor necrosis factor (TNF)-α, transforming growth factor (TGF)-β1 production did not show any differences. In addition, Foxp3 mRNA expression was comparable amongst all groups. In conclusion, we observed an inhibitory effect in T cell proliferation in response to multiple MHC mismatched alloantigens in MLR, and this effect might be associated with the upregulation of IL-10 expression.