Tissue-specific infection dynamics of male-killing and nonmale-killing spiroplasmas in Drosophila melanogaster
Tissue-specific infection dynamics of male-killing and nonmale-killing spiroplasmas in Drosophila melanogaster
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DOI：
10.1111/j.1574-6941.2006.00087.x
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发表时间：
2006-07-01
影响因子：
4.2
通讯作者：
Fukatsu, Takema
中科院分区：
文献类型：
--
作者：
Anbutsu, Hisashi;Fukatsu, Takema
由于雄性后代在胚胎发育过程中选择性死亡，杀死雄性的螺旋体菌株NSRO导致宿主黑腹果蝇的性别比例极度偏向雌性。螺原体菌株NSRO- a是NSRO的一种变体，不会引起这些症状。为了深入了解共生体诱导生殖表型的机制，利用定量PCR技术监测了宿主衰老过程中不同组织中螺旋体的感染密度。血淋巴的密度动态与全身相似，而脂肪体、肠和卵巢的密度动态与全身不同。这些结果表明，大多数螺旋体在宿主的血淋巴中定植和增殖。在血淋巴和全身中，NSRO的感染密度普遍高于NSRO- a，这可能与螺旋体引起的生殖表型不同有关。
The male-killing spiroplasma strain NSRO causes an extremely female-biased sex ratio of the host, Drosophila melanogaster, as a result of selective death of male offspring during embryogenesis. The spiroplasma strain NSRO-A, a variant of NSRO, does not cause such symptoms. In an attempt to gain insights into the mechanism underlying the symbiont-induced reproductive phenotype, infection densities of the spiroplasmas in different tissues were monitored during host aging using a quantitative PCR technique. The density dynamics in the hemolymph were reminiscent of those in the whole body, whereas the density dynamics in the fat body, intestine and ovary were not. These results suggest that the majority of the spiroplasmas colonize and proliferate in the hemolymph of the host. In the hemolymph and whole body, the infection densities of NSRO were generally higher than those of NSRO-A, which may be related to the different reproductive phenotypes caused by the spiroplasmas.