Thermosynechococcus switches the direction of phototaxis by a c-di-GMP dependent process with high spatial resolution
Thermosynechococcus switches the direction of phototaxis by a c-di-GMP dependent process with high spatial resolution
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热聚球藻通过具有高空间分辨率的 c-di-GMP 依赖性过程来切换趋光性方向
DOI：
10.1101/2021.08.26.457869
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
T. Nishizaka
中科院分区：
文献类型：
--
作者：
Daisuke Nakane;Gen Enomoto;A. Wilde;T. Nishizaka
许多蓝细菌通过光合作用将光作为能量来源，显示出朝向或远离光源的定向运动。然而，改变运动方向的分子和细胞生物学机制仍不清楚。在这里，我们利用光学显微镜观察了棒状嗜热蓝藻热共生球菌在生理温度和光照条件下的IV型菌毛依赖细胞运动。正、负趋光性控制在1 min的短时间尺度内。细胞在固体表面平滑地向绿光方向移动，但是当我们施加额外的蓝光照明时，细胞的方向被转换为向后移动。该开关由三种光感受器SesA、SesB和SesC介导，这三种光感受器具有蓝藻色素光感觉结构域和细菌第二信使环二聚体GMP （c-di-GMP）的合成/降解活性。我们的研究结果表明，趋光性方向转换的决策过程涉及c-二- gmp细胞浓度的光依赖性变化。此外，我们发现杆状细胞可以垂直于光矢量移动，这表明极性不仅可以通过极对极调节来控制，而且可以在一极内调节。这项研究提供了以前未描述的通过高空间分辨率的第二信使信号快速细菌极性调节的见解。
Many cyanobacteria, which use light as an energy source via photosynthesis, show directional movement towards or away from a light source. However, the molecular and cell biological mechanisms for switching the direction of movement remain unclear. Here, we visualized type IV pilus-dependent cell movement in the rod-shaped thermophilic cyanobacterium Thermosynechococcus vulcanus using optical microscopy at physiological temperature and light conditions. Positive and negative phototaxis were controlled on a short time scale of 1 min. The cells smoothly moved over solid surfaces towards green light, but the direction was switched to backward movement when we applied additional blue light illumination. The switching was mediated by three photoreceptors, SesA, SesB and SesC, which have cyanobacteriochrome photosensory domains and synthesis/degradation activity of the bacterial second messenger cyclic dimeric GMP (c-di-GMP). Our results suggest that the decision-making process for directional switching in phototaxis involves light-dependent changes in the cellular concentration of c-di-GMP. Furthermore, we reveal that rod-shaped cells can move perpendicular to the light vector, indicating that the polarity can be controlled not only by pole-to-pole regulation but also within-a-pole regulation. This study provides insights into previously undescribed rapid bacterial polarity regulation via second messenger signalling with high spatial resolution.
DOI：
10.1073/pnas.1812871115
发表时间：
2018-12
期刊：
Proceedings of the National Academy of Sciences
影响因子：
--
作者：
Yiling Yang;Vinson Lam;M. Adomako;Ryan Simkovsky;A. Jakob;N. Rockwell;Susan E. Cohen;Susan E. Cohen;A. Taton;Jingtong Wang;J. Lagarias;A. Wilde;David Nobles;J. Brand;S. Golden
通讯作者：
Yiling Yang;Vinson Lam;M. Adomako;Ryan Simkovsky;A. Jakob;N. Rockwell;Susan E. Cohen;Susan E. Cohen;A. Taton;Jingtong Wang;J. Lagarias;A. Wilde;David Nobles;J. Brand;S. Golden
DOI：
10.1073/pnas.1621469114
发表时间：
2017-01
期刊：
Proceedings of the National Academy of Sciences
影响因子：
--
作者：
B. Sabass;Matthias D. Koch;Guannan Liu;H. Stone;J. Shaevitz
通讯作者：
B. Sabass;Matthias D. Koch;Guannan Liu;H. Stone;J. Shaevitz
DOI：
10.1073/pnas.2023988118
发表时间：
2021-03-23
影响因子：
11.1
作者：
Harwood, Thomas, V;Zuniga, Esthefani G.;Risser, Douglas D.
通讯作者：
Risser, Douglas D.