Organization of stem cells and their progeny in human epidermis
Organization of stem cells and their progeny in human epidermis
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DOI：
10.1111/j.0022-202x.2004.23599.x
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发表时间：
2005-02-01
影响因子：
6.5
通讯作者：
Taichman, LB
中科院分区：
文献类型：
--
作者：
Ghazizadeh, S;Taichman, LB
表皮的更新是通过干细胞和转运扩增细胞的有序复制和终末分化来实现的。在小鼠表皮中，更新是围绕被称为表皮增殖单位（EPU）的高度有序结构组织的，每个EPU由单个干细胞产生。将这些概念应用于人体表皮是很困难的，因为人体表皮的基底层是起伏的，地层的厚度是可变的。为例。目前尚不清楚人表皮的干细胞是位于表皮嵴的基部还是位于真皮乳头的顶端。数据支持这两种观点。为了更好地了解EPU在人类皮肤中的组织，我们用编码荧光标记蛋白的慢病毒对人类包皮异种移植进行了遗传标记，并在28周内绘制了标记细胞柱。通过跟踪这些柱在表皮中的起源，我们已经能够确定干细胞沿着基底室分散。这些柱的宽度确实变化很大，最窄的起源于位于远脊底部的细胞。这些发现为人类无毛皮肤表皮更新动力学提供了新的见解。
Renewal of epidermis is achieved by an ordered replication of stem cells and transit amplifying cells followed by terminal differentiation. In mouse epidermis, renewal is organized around highly ordered structures termed epidermal proliferative units (EPU), each generated by a single stem cell. It has been difficult to apply these concepts to the human epidermis where the basal layer is undulating and the strata have variable thickness. For example. it is unclear whether stem cells in human epidermis are located at the base of rete ridges or overlying the tip of dermal papilla. Data are available to support both views. To gain a better understanding of EPU organization in human skin, we have genetically marked xenografts of human foreskin with a lentivirus encoding a fluorescent marker protein and have mapped labeled columns of cells over a 28-wk period. By following these columns to their origin in the epidermis we have been able to determine that stem cells are dispersed along the basal compartment. The widths of these columns do vary considerably, with the narrowest originating from cells located in the base of the rete ridge. These findings provide new insights into the dynamics of epidermal renewal in human glabrous skin.