Comparative high-resolution mapping of human and primate chromosomes by fluorescence in situ hybridization.
Comparative high-resolution mapping of human and primate chromosomes by fluorescence in situ hybridization.
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DOI：
10.1006/geno.1993.1479
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发表时间：
1993-11
期刊：
影响因子：
4.4
通讯作者：
T. Ried;N. Arnold;D. Ward;J. Wienberg
中科院分区：
文献类型：
--
作者：
T. Ried;N. Arnold;D. Ward;J. Wienberg
一种分子细胞遗传学的方法，有利于高分辨率的比较映射定义的人类基因在不同的灵长类动物物种。荧光原位杂交和数字成像显微镜被用来可视化人类DNA探针上同时带或“画”的中期染色体的类人猿（黑猩猩，大猩猩，Pongo pygmaeus），hylobatids（Hylobates lar和Hylobates syndactylus），和旧大陆猴（猕猴fuscata和Cercopithecus aethiops）。利用一系列DNA探针，在分子细胞遗传学水平上很容易检测到灵长类动物核型中的染色体重排。这一方法将大大有助于理解灵长类动物的生殖和进化。
A molecular cytogenetic approach that facilitates high-resolution comparative mapping of defined human genes in different primate species is presented. Fluorescence in situ hybridization and digital imaging microscopy were used to visualize human DNA probes on simultaneously banded or "painted" metaphase chromosomes of great apes (Pan troglodytes, Gorilla gorilla, Pongo pygmaeus), hylobatids (Hylobates lar and Hylobates syndactylus), and Old World monkeys (Macaca fuscata and Cercopithecus aethiops). Using a series of DNA probes, chromosomal rearrangements in the karyotypes of primates were readily detected at the molecular cytogenetic level. This approach should contribute considerably to the understanding of primate phylogeny and evolution.